Research Articles

2018  |  Vol: 4(2)  |  Issue: 4(March-April)  |
In vitro anti inflammatory activity of unpurified and purified Manahshila

Vineet Sharma1, Himanshu2, Dev Nath Singh Gautam1*

1Department of Rasa Shastra, Faculty of Ayurveda, Institute of Medical Sciences, Banaras Hindu University, Varanasi, U.P.,  India

2Department of Science and Technology-CIMS, Institute of Sciences, BHU, Varanasi, U.P., India

*Address for correspondence

Dev Nath Singh Gautam

Department of Rasa Shastra, Faculty of Ayurveda,

Institute of Medical Sciences, Banaras Hindu University, Varanasi, U.P.  India


Objective: Realgar (arsenic sulphide), popularly known as Manahshila (in India),  used for the treatment of various diseases like eye, skin, respiratory, psychological disorders and also including pain related ailments. This study is aimed to evaluate the anti-inflammatory efficacy of Ashodhita Manahshila (AM) and Shodhita Manahshila (SM) using in vitro models of inflammation. Material and methods: Shodhana (Purification) of Manahshila was carried out by seven bhavana (levigation) with ginger juice. Five concentration levels (20, 40, 60, 80 and 100 µg/mL) of the AM, SM were evaluated by using heat induced protein denaturation and heat induced hemolysis of the erythrocyte and subjected to determination of absorbance to assess the anti-inflammatory activity. Indomethacin was used as the positive control at same concentration levels. Results: The present findings exhibited a concentration dependent inhibition of protein denaturation and hemolysis of the erythrocyte by the SM. The effect of AM was found to be less when compared with the SM and indomethacin. From the present study it can be concluded that SM marked in vitro anti-inflammatory effect as compared to AM against the denaturation of protein and hemolysis of the erythrocyte. Conclusion: By this study it can be concluded that after Shodhana, drug not only transformed into physical and chemical changes but also safely digestible as well as improve therapeutic efficacy.

Keywords: indomethacin, Manahshila, protein denaturation, Shodhana

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