Research Articles

2019  |  Vol: 5(5)  |  Issue: 5(September- October)  |  https://doi.org/10.31024/ajpp.2019.5.5.17
In vitro and in vivo neutralizing potential of Alangium salvifolium extract against Russell’s viper (Daboia russelli)

Movalia Dharmistha1*, Manek Ravi2, Dudharejiya Ashvin2

1Department of Pharmacognosy, S. J. Thakkar Pharmacy College, Rajkot (Gujarat) India

2Department of Pharmacognosy, B. K. Mody Govt. Pharmacy College, Rajkot (Gujarat) India

*Address for Corresponding Author

Movalia Dharmistha

Department of Pharmacognosy,

S. J. Thakkar Pharmacy College, Rajkot (Gujarat) India

Abstract

Objective: The objective of the work is to determine the Russell viper venom neutralization efficiency of the fraction of methanolic extracts of Alangium salvifolium by in vitro and in vivo methods. Material and methods: In the present study, the anti-snake venom activity was investigated by using different in vitro methods viz., phospholipase inhibition activity and Neutralization of Coagulant activity of venom. The ethyl acetate fraction was subjected to in vivo study by Neutralization of lethal effect (ED50) of Venom, Neutralization of haemorrhagic activity and Neutralization of mast cell degranulation. Results and conclusion: Different doses of fractions were effective against Russell’s viper venom in a concentration dependant manner. But it was found that ethyl acetate fraction at a dose of 400 µg inhibited greater than half of the halos 4.33 mm (68.21%) in phospholipase inhibition activityand showed a high significant (P<0.001) reduction in the coagulant activity with time period of 144.33±1.51s (77.73%). Ethyl acetate fraction showed potent snake venom neutralizing capacity against Russell viper venom in Albino wistar rats and swiss albino mice in dose dependent manner.100% neutralization of median lethal dose (LD50) 0.6µg/g of venom was observed in 500mg/kg of ethyl acetate fraction treated animals. Ethyl acetate fraction (500 mg/kg) showed significant decrease in haemorrhagic lesions (81% inhibition) and inhibit venom induced mast cell degranulation (49.52% protection). The results are comparable to Standard antisnake venom (ASV).

Keywords: Alangium salvifolium, anti-snake venom, Russell’s viper venom

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