Yessian Sharmila Juliet, Kandasamy Kalimuthu*, Vajjiram Chinnadurai, Venkatachalam Ranjitha, Ammasai Vanitha
Plant Tissue Culture Division, PG and Research Department of Botany,
Government Arts College (Autonomous), Coimbatore-641018, India.
*Address for Corresponding Author
Dr. K. Kalimuthu, Assistant Professor,
PG and Research Department of Botany, Government Arts College (Autonomous),
Coimbatore ‑ 641 018, Tamil Nadu, India
Objective: Ethanol and methanol extracts of wild and tissue cultured Cadaba fruticosa were studied and compared for its antimicrobial activity against six human pathogenic organisms and anticancer activity against HeLa cancer cell lines. Materials and Methods: Antimicrobial activity of Wild Cadapa fruticosa ethanol, Micropropagated/ tissue cultured plant ethanol, Wild Cadapa fruticosa methanol, Micropropagated/tissue cultured plant methanol (WCFE, MCFE, WCFM and MCFM) plant extracts were investigated by well diffusion susceptibility method and also in vitro cytotoxicity activity was studied by MTT assay at different concentration. Results and Conclusion: The results showed that the highest zone of inhibition was obtained in Escherichia coli (14±0.82 mm and 08±1.05mm) at 60 µl concentration of wild and tissue cultured Cadaba fruticosa ethanol extracts. Whereas in methanol extract the highest zone of inhibition (14±0.14 mm and 09±0.12 mm) in 60 µl concentration against Streptococcus pyogenes and Staphylococcus aureus. In both the cases the activity of the extract was less against fungal pathogens. The higher percentage of anticancer activity was observed in wild and tissue cultured Cadaba fruticosa of ethanol extracts with 53.14 and 54.78 at 5 mg/ml concentration. Whereas in wild and tissue cultured Cadaba fruticosa methanol extracts treatment were 52.58 and 51.39 respectively. The percentage of inhibition was increased with the increasing concentration. The IC50 value of wild and tissue cultured Cadaba fruticosa ethanol extracts were 260.36 mg/ml and 268.05 mg/ml, methanol extracts were 273.71 mg/ml and 286.73 mg/ml respectively. Results of this study are important because these results confirmed the use of tissue cultured plants instead of natural plants. However more investigation are needed to identity the active principles and its molecular mechanism to explain their therapeutic efficacy.
Keywords: Cadaba fruticosa, Streptococcus, HeLa cancer, tissue cultured plant