Research Articles

2019  |  Vol: 5(3)  |  Issue: 3 (May- June)  |
Study on the extraction and purification of metabolites obtained from Trichoderma harzianum

Bina Pani Gupta1*, Abhishek Mathur2, Akshma Koul2, Vandana Shrivastava3

1Dept. of Microbiology, Himalayan University, Arunachal Pradesh, India

2Dept. of Research & Development, NCS Green Earth Pvt. Ltd., Nagpur, Maharashtra, India

3Dept. of Microbiology, Subharti University, Meerut (U.P), India

*Address for corresponding author:

Bina Pani Gupta

Dept. of Microbiology

Himalayan University, Arunachal Pradesh, India


Background: Trichoderma are closely related with their ability to produce a wide range of lysing enzymes, to degrade substrates and to possess high resistance to microbial inhibitors. Trichoderma sp. include a number of fungal strains that are used as biocontrol agents due to their abilities to antagonize a wide range of phyto-pathogenic fungi, bacteria and oomycetes, through several mechanisms that are activated in Trichoderma by the pathogens. Trichoderma sp. antagonizes phyto-pathogens by competing for nutrients, space, by producing antibiotics as well as by inducing systemic resistance of plants. Objectives: The aim of present study was to identify, extraction and purification of secondary metabolites from Trichoderma harzianum Materials and methods: In the present investigation, Trichoderma isolate was isolated and purified from different soil samples. Further, the extraction and purification of secondary metabolites was performed by using ethyl acetate as a solvent. The solvent extract was dried in order to obtain the crude extract which is further processed for GC-MS characterization.  Results: The GC/MS analysis of ethyl acetate extract revealed the presence of 12 compounds. These compounds were determined as 4 volatile alcohols (1-4) and fatty acid esters (5-12). The mixtures of secondary metabolites were further evaluated against the pathogenic strains for determination of antimicrobial spectrum.

Keywords: Trichoderma harzianum, secondary metabolites, compounds, antimicrobial activity

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