Research Articles

2019  |  Vol: 5(3)  |  Issue: 3 (May- June)  |  https://doi.org/10.31024/ajpp.2019.5.3.16
Study on the extraction and purification of metabolites obtained from Trichoderma harzianum

Bina Pani Gupta1*, Abhishek Mathur2, Akshma Koul2, Vandana Shrivastava3

1Dept. of Microbiology, Himalayan University, Arunachal Pradesh, India

2Dept. of Research & Development, NCS Green Earth Pvt. Ltd., Nagpur, Maharashtra, India

3Dept. of Microbiology, Subharti University, Meerut (U.P), India

*Address for corresponding author:

Bina Pani Gupta

Dept. of Microbiology

Himalayan University, Arunachal Pradesh, India

Abstract

Background: Trichoderma are closely related with their ability to produce a wide range of lysing enzymes, to degrade substrates and to possess high resistance to microbial inhibitors. Trichoderma sp. include a number of fungal strains that are used as biocontrol agents due to their abilities to antagonize a wide range of phyto-pathogenic fungi, bacteria and oomycetes, through several mechanisms that are activated in Trichoderma by the pathogens. Trichoderma sp. antagonizes phyto-pathogens by competing for nutrients, space, by producing antibiotics as well as by inducing systemic resistance of plants. Objectives: The aim of present study was to identify, extraction and purification of secondary metabolites from Trichoderma harzianum Materials and methods: In the present investigation, Trichoderma isolate was isolated and purified from different soil samples. Further, the extraction and purification of secondary metabolites was performed by using ethyl acetate as a solvent. The solvent extract was dried in order to obtain the crude extract which is further processed for GC-MS characterization.  Results: The GC/MS analysis of ethyl acetate extract revealed the presence of 12 compounds. These compounds were determined as 4 volatile alcohols (1-4) and fatty acid esters (5-12). The mixtures of secondary metabolites were further evaluated against the pathogenic strains for determination of antimicrobial spectrum.

Keywords: Trichoderma harzianum, secondary metabolites, compounds, antimicrobial activity

Manuscript Management System
Submit Article Subscribe Most Popular Articles Join as Reviewer Email Alerts Open Access
Our Another Journal
Another Journal
Call for Paper in Special Issue on

Call for Paper in Special Issue on