Abubakar Dabo Dalhat1*, Dalha Wada Taura2, Dambazau Ado Musa2, Sadiya Suleiman Ayuba2, Musayyiba Shu’aibu2, Adam Uba Muhammad
1Department of Biotechnology, Faculty of Life Science Mewar University, Gangrar, Chittorghar (RAJ) India.
2Department of Microbiology Faculty of Life Science Bayero University Kano PMB: 3011
* Address for Corresponding Author
Abubakar Dabo Dalhat
Department of Biotechnology, Mewar University,
Gangrar, Chittorghar (RAJ) India
Abstract
Background: Medicinal plants have been used in traditional medicine practice since ancient time. Hundreds of chemical compounds which offer defence against insects, bacteria, fungi, diseases and herbivorous mammals are synthesised by these plant. Objectives: The objective of present study was to determine the antibacterial activity of some plants used by herbalist to treat wound and urinary tract infections. Material and Methods: Aqueous and methanol extracts of leaves of three important medicinal plant species, Momordica charantia, Ocimum sanctum and Prosopis juliflora has been tested individually for their antibacterial activity against Staphylococcus aureus, Streptococcus pneumonia, Pseudomonas aeruginosa, Escherichia coli, klebsiella pneumoniae and Streptococcus pyogens isolated from wound and urine using agar well diffusion method. Results and conclusion: On the basis of results obtained from the preliminary antibacterial activity, aqueous and methanolic leaf extracts while tested individually showed various degrees of activity. Methanolic extract was found to be the most active in respect to the wide range of inhibition zones against all test bacteria. The methanolic leaves extracts of M. charantia showed comparatively a high degree of activity followed by P. juliflora and O. sanctum. The diameter of ZI was 21mm for methanolic extract of M. charantia extract against S. pyogens. The least activity was observed against P. aeruginosa showing 7 mm and 8 mm by methanolic and aqueous leaf extract of O. sanctum at 50 mg/ml.
Keywords: Momordica charantia, Ocimum sanctum, Prosopis juliflora. Zone of inhibition, antibacterial
