Sanchita Bhattacharya1,2*, Sanjit Debnath2, Panna Das3, Ajay Krishna Saha2
1Department of Botany, M.B.B. College, Agartala -799004, Tripura West, India
2Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Suryamaninagar-799022, Tripura, India
3Microbiology Laboratory, Department of Botany, Tripura University, Suryamaninagar-799022, Tripura, India
*Address for Corresponding Author:
Department of Botany, M.B.B. College, Agartala -799004, Tripura West, India
Background: Diversity of fungal communities may differ between and within organs and tissues of an individual plant. Plants as an important source of biologically active compounds and thus are used in traditional healing practices. Some fungi, isolated from the host plant are also co-producer of active metabolites. Objective: Present study documented the distribution of fungal endophytes in green leaf and root explants from Ananus comosus var. kew in fruiting and non-fruiting season. Materials and methods: Three Experimental sites were randomly selected for sampling of explants from Unokoti District, Tripura. Isolation and identification of fungal endophytes were done. Cultivation and extraction of Neopestalotiopsis piceana (isolated from both leaf and root) was done for determination antioxidant and antibacterial activity. Malt extract broth (MEB) and Potato dextrose broth (PDB) were used as growth media and ethyl acetate and methanol were used as extraction solvent. Results: Total twenty four endophytic fungal strains and four non-sporulating forms were isolated. Highest relative frequency was recorded in Pseudopestalotiopsis theae from leaf, Trichoderma asperellum from root and Neopestalotiopsis piceana from both leaf and root endophytes. Lowest EC50 was recorded in case of ethyl acetate extract of mycelial mat harvested from PDB. Maximum inhibition zone was obtained for ethyl acetate extracted mycelial mat harvested from PDB growth medium against Staphylococcus aureus. Conclusion: Growth media and type of extraction solvent affect the secondary metabolite production in fungal strain. This is the first report of N. piceana, isolated from A. comosus of Tripura and hence its bioactive potential was evaluated.
Key words: Antimicrobial activity, broth extract, DPPH activity, mycelial extract