Research Articles

2016  |  Vol: 2(3)  |  Issue: 3 (May- June)
Evaluation of anthelmintic and antimicrobial activity of Ursolic acid obtained from Tulsi (Ocimum sanctum)

Prakash Pandey1, Ashish Garg2, Vishal Singh2, Ajay Shukla3

1Department of Chemistry and Pharmacy, Rani Durgavati University, Jabalpur, India

2Department of Pharmaceutical Science, Guru Ramdas Khalsa Institute of Science & Technology Pharmacy, Jabalpur, India

3Department of Pharmaceutical Science, Mohanlal Sukhadia University, Udaipur Rajasthan, India

*Address for Corresponding Author:

Mr. Ashish Garg

Department of Pharmaceutical Science,

Guru Ramdas Khalsa Institute of Science & Technology Pharmacy,

Jabalpur, India

Abstract

Objective: This study was designed to extract ursolic acid (UA) from Ocimum sanctum and to evaluate the anthelmintic activity and antimicrobial effects on the E coli. Ursolic acid, an important bioactive compound, was isolated from ethanolic extract of Ociumum tenuiflorum plant also known as Ocimum sanctum. Materials and methods: Ursolic acid was isolated from the ethanolic extract of plant Ocimum sanctum. It was evaluated for anthelmintic activity and antibacterial action on E. coli with disc diffusion and well diffusion method. Compare the anthelmintic activity and antibacterial action of ursolic acid with standard drug albendazole and tetracycline respectively. Results: The evident obtained from the experimental data, ursolic acid showed significant anthelmintic activity against earthworm Pheretima postuma. Screening of compound was performed for complete death and paralysis of worms. The results reveal a dose dependent increase in activity of the compound at 15, 20 and 25 mg/ml concentration. Results were comparable along the standard drug albendazole, in the same concentration. The results of antibacterial screening assay of ursolic acid by disk diffusion method and well diffusion method. The in vitro anti-microbial studies reveal that the ursolic acid was potentially active against microbial species and exhibit greater biocidal effect as compared to ligand fragments. Conclusion: The present study shows the potent anthelmintic as well as antibacterial activity of ursolic acid that was good in their action against the worms. The results indicated that ursolic acid have the potential to paralyze and kill the parasitic worms.

Keywords: Ocimum sanctum, ursolic acid, anthelmintic activity, antibacterial activity

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