Research Articles

2019  |  Vol: 5(2)  |  Issue: 2(March-April)  |
Qualitative and quantitative phytochemical analysis of Cassia hirsuta seeds

Mallappa Hanamantappa Shalavadi1*, Chandrashekhar Venkaraddi Mangannavar1, Iranna S. Muchchandi 1, Basavaraj Hulakoti2

1Department of Pharmacology, BVVS’s Hanagal Shri Kumareshwar College of Pharmacy, Bagalkot-587101, Karnataka, India

2Department of Pharmacology, Government College of Pharmacy, Bengaluru, Karnataka, India

*Address for Correspondence Author:

Mallappa Hanamantappa Shalavadi,

Assistance Professor,

Department of Pharmacology, HSK College of Pharmacy, Bagalkot-587101,

Karnataka, India.


Objective: The present study aim was to analyze quantitatively and qualitatively the phytochemicals as well as the investigating the physicochemical properties from seeds of Cassia hirsuta and its chloroform and ethanol extracts. Materials and Methods: In this the physico-chemical evaluation, ash values viz., total ash, acid insoluble ash and water soluble ash, sulphated ash, and extractive values viz., alcohol soluble extractive value, water soluble extractive and chloroform soluble extractive value, and loss on drying were determined. Preliminary phytochemical screening where done for presence of carbohydrates, proteins and amino acids, glycosides, alkaloids, phytosteroids, flavonoids, saponins and tannins and phenolic compounds, and total phenolic content was estimated by Folin Ciocalteu’s method and total flavonoid content was measured with the aluminium chloride colorimetric assay. Results: In results it was found that the seed contains various phytochemicals were present in its ethanol and chloroform extracts. The total phenolic content in the ethanol and chloroform extracts were found 13.7±0.4187 and 7.367±0.2987 mg of gallic acid equivalent weight/g of extract respectively and the concentration of flavonoids in plant ethanol and chloroform extracts of Cassia hirsuta were found 114.6±13.33 and 99.56±11.6 mg of quercetin equivalent weight/ g of extract respectively. Conclusion: The present study concludes that the seeds of Cassia hirsuta have the potential to act as a source of useful drugs because of presence of various phytochemical constituents and high concentration of phenolic and flavonoid compounds.

Keywords: Cassia hirsuta, ash values, flavonoids, phenols, phytochemicals

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