Research Articles

2018  |  Vol: 4(2)  |  Issue: 4(March-April)  |  https://doi.org/10.31024/ajpp.2018.4.2.19
Effect of Quercetin on Beta cell Regeneration

Mayuri Gurav*, Satish Bhise, Snehal Warghade              

Smt. Kashibai Navale College of Pharmacy (Savitribai Phule Pune University), Kondhwa, Pune 411048, M.S., India.

*Corresponding author

Mayuri V. Gurav

Department of Pharmacology, Smt. Kashibai Navale College of Pharmacy, Kondhwa, Pune 411048, M.S., India.

Abstract

Objective: The aim of the present study was to investigate the role of Quercetin in beta cell regeneration in vitro and in vivo. Methods: The research work was initiated with in vitro experiment wherein 3-(4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide (MTT) assay was performed using the MIN6 cell line. In vivo study was performed in Streptozocin-induced diabetic Wistar rats and Quercetin (QE) was administered orally in three doses (25, 50, 100 mg/kg). Body weights, serum insulin, blood glucose were measured. At the end of the study animals were sacrificed and histological examination was carried out which included normal histopathology, immunohistochemistry and 5-Bromo-2'-deoxyuridine (BrdU) cell proliferation assay. Results: Streptozocin damaged MIN6 cells showed significantly (P < 0.001) higher viabilities after administration of QE (10μg/ml). QE administration significantly (P < 0.0001) increased body weights as compared to Diabetic Control (DC) group. Administration of QE (50mg/kg) and QE (100mg/kg) significantly (P < 0.0001) decreased fasting blood glucose level and significantly (P < 0.001), (P < 0.0001) increased serum insulin level as compared to DC group. Pancreatic Insulin secretion significantly (P < 0.0001) increased in QE (100mg/kg) group as compared to DC group, also restoration of islets, reduced pancreatic damage with increase in number of β-cells was observed in QE (100mg/kg) group as compared to DC group.  The increased number of BrdU positive cells was observed on QE (100mg/kg) administration as compared to DC group. Conclusion: The present study thus confirmed beta cell regeneration using QE.

Keywords: Streptozocin, antibody, diabetes, insulin, MIN6

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