Mayuri Gurav*, Satish Bhise, Snehal Warghade
Smt. Kashibai Navale College of Pharmacy (Savitribai Phule Pune University), Kondhwa, Pune 411048, M.S., India.
Mayuri V. Gurav
Department of Pharmacology, Smt. Kashibai Navale College of Pharmacy, Kondhwa, Pune 411048, M.S., India.
Objective: The aim of the present study was to investigate the role of Quercetin in beta cell regeneration in vitro and in vivo. Methods: The research work was initiated with in vitro experiment wherein 3-(4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide (MTT) assay was performed using the MIN6 cell line. In vivo study was performed in Streptozocin-induced diabetic Wistar rats and Quercetin (QE) was administered orally in three doses (25, 50, 100 mg/kg). Body weights, serum insulin, blood glucose were measured. At the end of the study animals were sacrificed and histological examination was carried out which included normal histopathology, immunohistochemistry and 5-Bromo-2'-deoxyuridine (BrdU) cell proliferation assay. Results: Streptozocin damaged MIN6 cells showed significantly (P < 0.001) higher viabilities after administration of QE (10μg/ml). QE administration significantly (P < 0.0001) increased body weights as compared to Diabetic Control (DC) group. Administration of QE (50mg/kg) and QE (100mg/kg) significantly (P < 0.0001) decreased fasting blood glucose level and significantly (P < 0.001), (P < 0.0001) increased serum insulin level as compared to DC group. Pancreatic Insulin secretion significantly (P < 0.0001) increased in QE (100mg/kg) group as compared to DC group, also restoration of islets, reduced pancreatic damage with increase in number of β-cells was observed in QE (100mg/kg) group as compared to DC group. The increased number of BrdU positive cells was observed on QE (100mg/kg) administration as compared to DC group. Conclusion: The present study thus confirmed beta cell regeneration using QE.
Keywords: Streptozocin, antibody, diabetes, insulin, MIN6