Arasan Elayaraja1*, Ramu Peddimounika2, Subramanyeswara Rao Massimukku Rohitha2, Pannerpandiyan Premkumar3
1Associate Professor, Kamalakshi Pandurangan College of Pharmacy, Ayyampalayam, Thiruvannamalai District, Tamilnadu state, India.
2Research Scholar, SIMS College of Pharmacy, Guntur, Andhra Pradesh State, India.
3Associate Professor, SIMS College of Pharmacy, Guntur, Andhra Pradesh State, India.
*Address for Correspondence
Dr. A. Elayaraja M. Pharm, Ph. D, BTA, HTA, (MTA),
Kamalakshi Pandurangan College of Pharmacy,
Ayyampalayam, Thiruvannamalai District, Tamilnadu state, India
Objective: The main objective of the present work is to screen the antioxidant and antibacterial activity of various extracts obtained from flowers of Calotropis gigantea belonging to the family Apocynaceae. Materials and Methods: Both organic (Pet ether, Benzene and Acetone) and aqueous (Hydroalcohol) extracts of Calotropis gigantea flowers were prepared by using Soxhlet and cold maceration technique respectively. The extracts were concentrated using rotary evaporator. By using invitro paradigms, DPPH, nitric oxide and hydroxyl free radical scavenging activity were carried out. Meanwhile all the extarcts were subjected for antibacterial activity using various gram+ive and gram-ive bacterias in the substrate medium of Muller agar media with Diffusion assay method. Results: In the antioxidant activity, Pet ether extract showed a significant activity than other extracts. Also increase in concentration of the extract showed an increase in inhibition of the generfated free radicals. At most the pet ether extract at a higher concentration of 320 mcg/ml showed an increased inhibition of DPPH (73.5±0.2%), Nitric oxide (56.1±0.3%) and Hydroxyl (78.1±0.8%). Meanwhile antibacterial activity of hydroalcohol extract showed an increased zone of inhibition for various organisms used in this method than other extracts and significant to the standard, Pefloxacin. Conclusion: It was investigated that antioxidant activity showed a potential inhibition on generated free radicals and both gram+ive and gram-ive bacterial strains. Further studies are needed to evaluate the structural elucidation of active ingredients present in those extracts.
Keywords: Calotropis gigantea, extraction, antioxidant, antibacterial