Avik Das1, Subhajit Hazra1, Shailendra Patil2, Kalyan Kumar Sen1
1Department of Pharmacology, Gupta College of Pharmaceutical Sciences, Asansol, West Bengal
2Department of Pharmacology, Faculty of Pharmacy, Swami Vivekanand University, Saugor (M.P.), India
*Address for Correspondence:
Department of Pharmacology
Gupta College of Pharmaceutical Sciences, Asansol, West Bengal, India
Background: Despite its widespread use in traditional medicine, availability of a report on the toxicity of stem bark extract of Terminalia tomentosa (Asan) is lacking largely. So the present study aims to provide an account of the acute toxic potential of the ethanolic extract of the dried stem bark of Terminalia tomentosa in accordance to OECD TG 425. Materials and Methods: Female mice were randomly assigned to either test or control groups (n = 5). The test animals received a single dose of 2000 mg/kg b.w. T. tomentosa bark extract while the control ones received vehicle of equal volume. Both the groups were kept under observation for a period of fourteen days for signs of mortality and morbidity. Blood samples were collected periodically through the observation period for assessing hematological and biochemical parameters. Animals were sacrificed at the end of the observation period and vital organs were collected for histo- pathological analysis. Results: The data presented is indicative of the conclusion that LD50 of the extract is beyond 2000 mg/kg and hence the extract under investigation can be considered practically non-toxic. The vital parameters fairly remained constant across the groups with no significant alterations observed in the animals of either groups with respect to ALT, total protein, globulin etc. though AST was slightly raised in the test groups with a dip in ALP. Hematological and biochemical parameters however didn’t show significant variation between the groups. Conclusion: Results suggest that LD50 of the extract is greater than 2000 mg/kg and the putative role of the same in hepatoprotection is hinted by the elevation of AST in the test group.
Keywords: Ulcerative colitis, Terminalia tomentosa, toxicity