Research Articles

2019  |  Vol: 5(5)  |  Issue: 5(September- October)  |
Phytochemical screening, in vitro antioxidant activity, cytotoxicity study using Brine shrimp and antimicrobial study of Acanthus ilicifolius (Linn.) leaves

Saliny Aiyer*, K. G. Manju

Department of Zoology, Mar Ivanios College, Trivandrum, 695015, India

*Address for Corresponding author:

Saliny Aiyer

Department of Zoology, Mar Ivanios College, Trivandrum, 695015, India


Background: Acanthus ilicifolius, an associate mangrove also known as Holly mangrove is a shrub abundantly found in marshy areas and coastal areas that are frequently inundated with sea water. The leaves of this shrub are widely used by tribal people for medicinal purposes mainly treating snake bites and for curing rheumatism, asthma, diabetes, hepatitis, osteoporosis and inflammation. Objective: The study is intended to explore the phytochemical, antioxidant, cytotoxic and antimicrobial efficiency of A. ilicifolius leaves scientifically. Materials and Methods: In this study the methanol, hexane and chloroform extract of leaves of A. ilicifolius were subjected to qualitative screening of phytochemicals and quantitative estimation of their antioxidant capacity and cytotoxicity study using brine shrimp larvae along with antimicrobial efficiency by well diffusion method were done. Results and Conclusion: During the phytochemical screening the leaf extract was found to be rich in bioactive compounds. The LC50 value (for methanol 537.16 ± 8.3 µg/ml, hexane 691.84 ± 1.85 µg/ml and chloroform 776.27 ± 4.18 µg/ml) derived from probit regression method showed that A. ilicifolius leaf has low toxicity towards Artemia nauplii. The leaf extract showed good antioxidant activity during hydroxyl radical scavenging assay (IC50 for methanol 169 µg/ml, chloroform 2310 µg/ml and hexane 4336.6 µg/ml) and total antioxidant activity test (for hexane 15.31 ±1.1 mg/g AAE, chloroform 20.24 ± 1.23 mg/g AAE and methanol 27.095 ± 2.17 mg/g AAE). Antimicrobial activity using well diffusion method showed that A. ilicifolius leaf extract is an effective antimicrobial agent with highest percentage inhibition against Salmonella (methanol and hexane extract) and Mycobacterium species (chloroform extract). The study establishes that Acanthus ilicifolius is potentially a good candidate for numerous medical applications.

Keywords: Acanthus ilicifolius, phytochemical, cytotoxicity, antioxidant, antimicrobial, Mycobacterium

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