R. Jaya Krishna, Abhishek Pandagale, P. M. Ronad, S. S. Honnalli, Bhrigu Kumar Das, Pramod C. Gadad*
KLE College of Pharmacy, Hubli, Karnataka, India (580031)
*Address for Correspondence
Dr. Pramod C. Gadad
Department of Pharmacology, KLE College of Pharmacy, Vidyanagar, Hubli, Karnataka, India (580031)
Objective: The present investigation involves the synthesis and examination of anti-inflammatory activity of schiff bases of 7-amino-4-methyl-coumarins in different experimentally induced in-vivo inflammatory models. Materials and methods: The compounds were synthesized by reaction of 7-amino-4-methyl-coumarin with ethyl chloroformate to obtain 7-urethane-4-methyl-benzopyran-2-one. This was further condensed with hydrazine hydrate to obtain N-(4-methyl-2-oxo-2H-chromen-7-yl) hydrazine carboxamide, which was refluxed with substituted aromatic aldehydes to form schiff base (IVa-c). The structure of the newly synthesized compounds was confirmed by spectral data. The in-vivo anti-inflammatory activity of synthetic compounds was carried out using acute anti-inflammatory models (Histamine, Carrageenan and Formalin induced paw oedema) and sub-acute anti-inflammatory model (Cotton pellet granuloma). Results and conclusion: The results showed that treatment with the coumarin derivatives (50 mg/kg) significantly inhibit the acute and sub-acute anti-inflammatory models when compared with the control treated rats. The present study suggests that the coumarin derivatives possess anti-inflammatory activity in different experimentally induced inflammatory models.
Keywords: Coumarin derivatives; anti-inflammatory; inflammatory mediators; diclofenac sodium