Research Articles

2019  |  Vol: 5(3)  |  Issue: 3 (May- June)  |  https://doi.org/10.31024/ajpp.2019.5.3.15
Protective role of Gymnema sylvestre leaf extract on high sucrose diet-induced diabetic like phenotype, oxidative stress, reproductive fitness and longevity in Drosophila melanogaster

Hassan Rangegowda Harshavardhana, Mysore Siddaiah Krishna*

Drosophila Stock Centre, Department of studies in Zoology, University of Mysore, Manasagangotri, Mysore 570006 India

*Address for Corresponding author

Prof. Mysore Siddaiah Krishna

Drosophila Stock Centre, Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore 570006 India

Abstract

Objective: Dietary components play important role in physiology, development and longevity of organisms. In the present study, D. melanogaster (fruit fly) was used to evaluate toxic effects resulted due to high sucrose diet such as phenotypic response, circulating metabolites, oxidative stress, reproductive traits and longevity. Material and methods: Flies of Drosophila melanogaster (w118) was raised in control diet,30% high sugar diet and treatment diet were subjected for lifespan (longevity) assay, body weight analysis, metabolites level, biochemical assays (ROS,SOD AND CAT) and male accessory gland  and sperm traits measurements. Results and conclusion: Adult fly fed with 30% of high sucrose diet showed increased levels of glucose, trehalose, triacylglycerol and hydrogen peroxide. In addition to this, the level of antioxidant enzymes SOD and CAT were significantly increased. The Gymnema sylvestre (GS) leaf extract treatment along with 30% high sucrose diet elicited reversal of above changes leading to normal phenotype, biochemical markers and reproductive traits in D.melanogaster. Thus the treatment of Gymnema sylvestre leaf extract counteracted the phenotypic abnormalities along with reduction in circulating metabolites to normal levels and over expressions of stress marker enzymes are blunted. Reproductive fitness parameter and longevity were positively reverted in the GS extract treated flies in D. melanogaster.

Keywords: Drosophila melanogaster, Gymnema sylvestre, longevity, oxidative stress, sucrose

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