Mithun Rudrapal*, Dipak Chetia
Dept. of Pharmaeutical Sciences, Dibrugarh University, Dibrugarh-786004, Assam, India
*Address for Corresponding Author
Department of Pharmaceutical Sciences
Dibrugarh University, Dibrugarh- 786 004 Assam, India
In this review, the laboratory protocol commonly employed for the biological evaluation of antimalarial activity of new drug substances including natural products has been detailed in a generalized, but concise form. The evaluation strategy covers a systematic and standardized methodology starting from in vitro cell-based screening to the in vivo assay method using animal models. These assay methods primarily focus on blood/ erythrocytic stages of the Plasmodium parasite, either in vitro (P. falciparum) or in vivo (P. yoelii) since this particular stage of the parasite mainly causes symptoms, manifestations and associated pathogenesis of the disease. This is the only parasitic stage in malaria disease that can be maintained in continuous blood cultures and also remains to be the prime target for most of the antimalarial drug molecules. A general approach to the antimalarial screening (in vitro and in vivo assay methods) routinely used for the sensitivity testing of newly developed antimalarial compounds is described herein.
Keywords: Malaria, antimalarial evaluation, P. falciparum, drug resistance, in vitro, in vivo