Saket Asati, Vandana Soni*
Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar-470 003, India
*Address for correspondence
Prof. Vandana Soni
Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar (M.P.) 470003, India
Objective: The drug targeting strategy is drawn more attention for the effective treatment of various types of lethal diseases such as, cancer. To improve the efficacy of drug the dual targeted delivery system was developed with the help of hyaluronic acid (HA) and RGD peptide. HA targets the overexpressed CD44 receptors while RGD peptide helps to target integrin (αvβ3) receptors on the tumor cells. Material and Methods: The drug, paclitaxel (Ptx); was used as the model drug and conjugated with HA and then the drug-HA conjugate was entrapped into the RGD decorated nanoparticulate system. The conjugation of the ligand molecules with the drug and carrier system was confirmed by the IR and NMR spectroscopy methods. Results: The developed NPs exhibited excellent physical properties like, particle size in nano size range, spherical shape, and high entrapment efficiency which may make drug delivery system more efficient. The cytotoxicity and cell uptake study of the prepared formulations on the cancer cell line (U87MG) (which has overexpressed integrin receptors, showed lowest IC50 value and highest cell uptake) showed better uptake of RGD decorated NPs due to receptor mediated transport across the cells. Conclusion: Thus, RGD peptide decorated system developed as the promising candidate for the treatment of cancer cells, effectively.
Keywords: RGD peptide, hyaluronic acid, targeted delivery system, integrin receptor, nanoparticles