Akanksha Malaiya, Awesh Kumar Yadav*
Drug Delivery and Nanotechnology Laboratories, Department of Pharmaceutics, Bhagyoday Tirth Pharmacy College, Sagar (MP), India.
*Address for Corresponding author
Dr. Awesh Kumar Yadav
Drug Delivery and Nanotechnology Laboratories,
Department of Pharmaceutics, Bhagyoday Tirth Pharmacy College Sagar (MP) India
Objective: The aim of present study was to evaluate the hyaluronic acid anchored chiosan nanoparticles containing polyphosphoric acid for the delivery of methotrexate. Material and methods: Chitosan nanoparticles with MTX were prepared by using ionotropic gelation technique. Preparation of hyaluronic acid (HA) anchored chitosan nanoparticles were prepared by ionic interaction method. NPs were exemplified by zeta potential, differential scanning calorimetry (DSC), particle size, field emission scanning electron microscopy, in-vitro release, entrapment efficiency, X-ray diffraction (XRD), polydispersity index, and stability studies at various temperature 5 ± 3ºC, 25 ± 2ºC and 40 ± 2ºC. Results: Studies demonstrated that HACSNPs have slower release (95.08% in 96 hrs) when contrasted with CSNPs (96.43% in 72hrs). Conclusion: The HACSNPs loaded with MTX were obtained successfully by ionotropic gelation method with high entrapment efficiency and low particle size. These formulations provide sustained and prolonged release of drug. Thus the NPs also hold a promising potential for treating the single or multiple ailments to improve patient compliance and reduce toxicity.
Keywords: Hyaluronic acid, Methotrexate, Chiotosan, Polyphosphoric acid, nanoparticles