Ruchi Dwivedi1, Namrata Dwivedi1, Pushpendra Shukla2, I.P. Tripathi1*
1Faculty of Sciences and Environment, MGCGV, Chitrakoot, Satna, M.P. India
2National Botanical Research Institute, Lucknow (M.P.), India
*Address for Corresponding Author
Prof. I. P. Tripathi
Faculty of Sciences and Environment, MGCGV, Chitrakoot, Satna, M.P. India
Objective: Evaluation and Quantification of Bioactive Metabolites in Bauhinia variegata Bauhinia variegata Linn. is well known herbal medicine in Ayurvedic and traditional system of medicine. Material and methods: Quantification of lupeol and oleanoleic acid have been done by HPTLC using solvent system toluene: ethyl acetate: formic acid (7:3:1). In-vitro antibacterial and antifungal activity of ethanol and water extract has been done, to evaluate the pharmacological potential of Bauhinia variegata. Results and conclusion: Lupeol and oleanoleic acid was resolved at Rf 0.76 ± 0.03 and 0.85 ± 0.04 by pre chromatographic derivatization with Anisyldehyde sulphuric acid. The amount of lupeol and oleanoleic acid was found to be 0.0007 and 0.0013 mg/ml in respectively. Considerable antioxidant activity in both the plants were observed showing IC50 values of 0.420±0.011 by DPPH method. As compared to ethanol extracts and water extracts of the plants showed stronger growth inhibitions against Salmonella, Staphylococcus, Pseudomonas, E. coli, Total coliform. Ethanolic extract are more effective compared to water extract. Plant derived medicines have made significant contribution towards human health and need to extend further for activity guided extraction/isolation of the active chemical moiety.
Keywords: Bauhinia variegata, HPTLC, Lupeol, Oleanoleic acid, DPPH, antimicrobial