Shampa Jain, Deepmala Batham*, Zubair Azad
Department of Zoology and Biotechnology, Govt. M.H. College of Home Science and Science for Women, Jabalpur (M.P.) India
*Address for Corresponding Author
Govt. Model Science College, Jabalpur (M.P.), India
Objective: In our present investigation the histopathological study was conducted in liver of Mystus cavasius, exposed to three sub-lethal concentration of a zinc sulphate after determination of LC50. Materials and methods: The healthy fish Mystus cavasius were exposed to sub-lethal concentrations of 2mg/L, 2.5mg/L and 3mg/L of zinc sulphate for 10 and 20 days. Liver from those fishes were taken and fixed in bouin's fluid for 24 hours for fixation. This fixed tissue was then processed through graded series of alcohols, cleared in xylene and embedded in paraffin wax. Sections of the paraffin wax were processed to obtain six micron thick paraffin sections and then stained with Hematoxylin and Eosin and examined under light microscope. Results: Histopathological changes revealed that the results observed in the liver includes degeneration of hepatic cells, vacuolar lesions, haemorrhages and ruptured blood vessels. Liver observed microscopically showed increasing degrees of damage in the tissues in correlation with the concentration of zinc sulphate. Conclusion: It can therefore be concluded that concentration of zinc sulphate caused histological alteration in the liver of Mystus cavasius.
Keywords: Histology, zinc sulphate, haemorrhages, liver, Mystus cavasius