Research Articles

2021  |  Vol: 7(1)  |  Issue: 1(January-February)  |
In vitro antioxidative and cytotoxic effect of protein fraction from Tabernaemontana divaricata leaves against DLA cells

M. Srilatha1*, K. Lavanya2, Subha T.1

1Department of Biotechnology, Sona College of Arts and Science, Salem - 636 005, Tamilnadu, India

2Department of Biochemistry, PSG College of Arts & Science, Coimbatore- 641014 Tamil Nadu India

*Address for Corresponding Authors

M. Srilatha

Department of Biotechnology, Sona College of Arts and Science, Salem - 636 005, Tamilnadu, India



Background: The rising burden of cancer worldwide calls for an alternative treatment solution. Herbal medicine provides a very feasible alternative to western medicine against cancer. Objectives: The present study assessed the in vitro antioxidative potential and cytotoxicity of Tabernaemontana divaricata leaves protein fraction. Materials and Methods: The antioxidant activity of protein fraction of T. divaricata was performed by several antioxidative assays including 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay, ABTS, superoxide radical (SO) scavenging assay and Hydrogen peroxide (H2O2) with standard protocol, followed by Cytotoxicity test with tryphan blue assay. Results: From the results, T.divaricata has been found to have the significant antioxidant activity in a dose-dependent manner and IC50 value was 59.1μg/ml for DPPH, 48 µg/ml for ABTS, 60 µg/ml for SO and 38.5 µg/ml for H2O2. Further, the cytotoxicity analysis was determined against Dalton Lymphoma Ascites (DLA) cell line and the IC50 value was found to be 62μg/ml for protein fraction of T.divaricata. Conclusion: Hence, the current study attests that T. divaricata is a fine source of natural antioxidants with anticancer agents and can be used in pharmaceutical preparations for the treatment of diseases induced by oxidative stress.

Keywords: T. divaricata, Antioxidants, Dalton Lymphoma ascites, Trypan blue assay

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