Research Articles

2017  |  Vol: 3(6)  |  Issue: 6 (November-December)
Effect of Couroupita guianensis on Xanthine Oxidase activity and its potent role in treatment of Gout

M. Syed Ali1*, V. Anuradha2, R. Keerthiga1, N. Yogananth1, H. Sheeba1

1PG and Research Department of Biotechnology, Mohamed Sathak College of Arts and Science, Sholinganallur, Chennai-600119, Tamilnadu, India

2PG and Research Department of Biochemistry, Mohamed Sathak College of Arts and Science, Sholinganallur, Chennai-600119, Tamilnadu, India

*Corresponding Author

M. Syed Ali

PG and Research Department of Biotechnology, Mohamed Sathak College of Arts and Science, Sholinganallur, Chennai-600119, Tamilnadu, India

Abstract

Objective: The aim of the present study was to evaluate the anti-inflammatory activity of fruit extract of Couroupita guinanensis. Materials and methods: In vitro anti-inflammatory activity was evaluated by using human red blood cell membrane stabilization studies. The inhibitory effect of Couroupita guianensis on Xanthine oxidase enzyme that promote the oxidation of hypoxanthine to xanthine which converts into uric acid, hence it is much effective in the treatment of gout arthritis. The ethanolic extract of Couroupita guinanensis was investigated for in vitro anti-inflammatory, albumin denaturation inhibitory activity, xanthine oxidase inhibitory activity. Total phenolics was quantified and bioactive compounds was identified by GC-MS analysis. Results: The ethanolic extract shows significant anti-inflammatory activity at the concentration of 400 μg/ml which is comparable to the standard drug 97.63 and fruit extract 79.97% The anti-inflammatory activity of the extracts were concentration dependent, with the increasing concentration the activity is also increased. Concentration of samples was observed at a concentration of 400ug/mL. The in vitro albumin denaturation inhibitory activity was compared with carried using Diclofenac, a standard anti-inflammatary drug which showed the maximum inhibition of 82.79% at the concentration of 400 μg/ml compared with control.. Conclusion: An enzyme xanthine oxidase inhibitory effect of Couroupita guianensis that promoteto the oxidation of hypoxanthine to xanthine which converts into uric acid, hence it is much effective in the treatment of gout arthritis. The ethanolic extract of the Couroupita guianensis fruit showed maximum xanthine oxidase inhibitory activity as compared to standard drug allopurinol.

Keywords: Anti-inflammatory, gout, Couroupita guianensis, Xanthine oxidase

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