Bushra Hasan Khan1*, Jameel Ahmad1, Farida Ahmad1, Syed Mobashir Yunus2
1Department of Pharmacology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India, 202002
2Department of Anatomy, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India, 202002
*Address for Corresponding author
Dr. Bushra Hasan Khan
Department of Pharmacology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, Uttar Pradesh, India, 202002
Objective: To evaluate the hepatoprotective effect of aqueous extracts of root (RAE) and peel (PAE) of Punica granatum. Materials and Methods: This study was conducted on adult albino Wistar rats of either sex weighing 150-200 g. Animals were divided into five groups (n=5). Liver injury was produced by carbon tetrachloride (CCl4) 1 ml/kg dissolved in olive oil (1:1) given intraperitoneally on day 1 and day 4 of the study duration of 14 days. Silymarin (50 mg/kg/d) orally was used as standard drug. Test groups received aqueous extract of P. granatum root (RAE) at doses of 200 and 400 mg/kg/day and aqueous extract of P. granatum (PAE) peel at doses of 200 and 400 mg/kg/day orally along with CCl4. On the 15th day, the hepatoprotective effect of RAE and PAE was evaluated by assessment of physical parameters, histopathological examination and biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and total serum bilirubin in blood sample. Results: The administration of RAE of P. granatum at doses of 200 and 400 mg/kg/day orally, exhibited a highly significant decrease in the rise of mean serum AST, ALT, ALP, and total bilirubin as compared to CCl4 treated group (p<0.001). PAE 200 mg/kg/day showed highly significant reduction in serum AST, ALT and serum total bilirubin (p<0.001). PAE 200mg/kg/day showed significant reduction in ALP (p<0.01). PAE 400mg/kg/day showed highly significant reduction in serum AST, ALT, ALP and total serum bilirubin when compared to CCl4 treated group (p<0.001). Histopathological examination of the liver also suggested hepatoprotective effect of RAE and PAE of P. granatum by restoration of hepatic architecture toward normal. Maximum decrease in the extent of centrilobular necrosis was observed in RAE 400 mg/kg/day treated rats when compared to CCl4 treated group. Conclusion: This study demonstrated hepatoprotective activity of RAE and PAE of P. granatum against CCl4 induced liver injury in rats.
Keywords: Punica granatum, Silymarin, hepatoprotective activity, Carbon tetrachloride