Priyanka Shrivastava1, Salaj Khare1*, B K Dubey2, Amit Joshi1, Amit Jain1, Suresh Dhakad1
1TIT- Pharmacy Education and Research, Anand Nagar, Bhopal, M.P., India
2Technocrats Institute of Technology- Pharmacy, Anand Nagar, Bhopal, M.P., India
TIT- Pharmacy Education & Research, Anand Nagar, Bhopal-21, (MP), India
Background: Diabetes mellitus mainly occurs due to deficiency or absence of insulin or rarely due to impairment of insulin activity causing varying degree of disruption of carbohydrate and fat metabolisum. Vitex negundo belonging to family Lamiaceae traditionally, used for treatment of muscle relaxant, pain reliving, anti-inflammatory, analgesic, and antiparasitic. Objective: This study will perform to investigate the hypoglycemic effect of stem extract of Vitex negundo. Material and methods: Hydro-alcoholic extraction of stem vitex negundo was prepared by soxhlet extraction method and futher subjected to acute oral toxicity animal received dose of 2000 mg/kg body weight, animals were observed individually for toxicity sign such as convulsion, depression mortality for 24hrs.Rat shall administer with alloxan monohydrate(120mg/kg)dissolved in sterile normal saline. When animal glucose level reach up to 200-260mg/100ml shall selected for experiment respectively. Selectively serum glucose, serum lipid and cholesterol level was determined and animal were divided into four groups. Group I (Test group) treated with stem extract (350 mg/kg)test drug, Group II(Positive control group)serve as diabetic control and were given only distilled water daily, Group III(Standard group) treated with glibenclamide (5 mg/kg) standard drug , Group IV ( normal control) only 0.5% CMC solution given daily. Results: Extract of Vitex negundo (stem) also significantly reduces blood glucose level in Alloxan induced diabetic rats and compare with standard glibenclamide. Histopathology study shows that normal control have minute edema, group second have pronounced edema. Conclusion: Thus, it can be concluded from above that Vitex negundo stem extract is found to be efficacious in reducing BGL and lipid levels.
Keywords: Diabetes mellitus, Vitex negundo, Alloxan, Glibenclamide, karyolysis, hypoglycemic