Research Articles

2017  |  Vol: 3(4)  |  Issue: 4 (July- August)
Isolation and HPLC quantitative analysis of flavonoids from flower extract of Punica granatum L.

R. Nalini*1, R. Anuradha2

1PG and Research Department of Biochemistry, Sengamala Thayaar Educational Trust Women’s College, Mannargudi, Tamilnadu, India -614001.

2PG and Research Department of Biochemistry, Sengamala Thayaar Educational Trust Women’s College, Mannargudi, Tamilnadu, India - 614001.

*Corresponding author

R. Nalini

PG and Research Department of Biochemistry,

Sengamala Thayaar Educational Trust Women’s College, Mannargudi, Tamilnadu, India -614001.

Phone No.:+91- 9003327926

Abstract                                                                                                                                            

Objective: The present study deals with the phytochemical screening of Punic granatum flowers, an important medicinal plant in all regions. This study involves the preliminary screening. Further HPLC flavonoid profile of the methanolic extract had been studied. Methods: The retention behavior of flavonoid isolated in methanolic extract of punica granatum flowers in high performance liquid chromatography (HPLC) system has been studied on an Inertsil C18-RP column using HPLC solvents were phosphate buffer (v/v) as aqueous solvent (A) and CH3CN as an organic solvent (B). Results: The preliminary screening test results in the detection of bioactive principles and the retention time is identified in the HPLC chromatogram, as flavonoid (Rt=21.8±0.14). Conclusion: The preliminary phytochemical analysis of plants will help in the identification of bioactive components present in each species, the knowledge of which could help in comparing the differences between species and also in drug discovery. The study will help in future for identifying this plant for further research.

Keywords: Phytochemicals, Punica granatum, flavonoid, HPLC

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