Research Articles

2020  |  Vol: 6(Articles in Press)  |  Issue: 3 (May-June)  |  https://doi.org/10.31024/ajpp.2020.6.3.3
Effects of DL-Methionine at various doses per se on liver in albino rats: An experimental study

Ervilla Dass1*, Swapan Goswami2, Maulin Mehta1

1Department of Pharmacology, Smt. Bhikhiben Kanjibhai Shah Medical Institute & Research Centre, Sumandeep Vidyapeeth an Institution Deemed to be University, At. & PO. Piparia, Tal. Waghodia, Dist. Vadodara -391760 (Gujarat), India

2Department of Pathology, Smt. Bhikhiben Kanjibhai Shah Medical Institute & Research Centre, Sumandeep Vidyapeeth an Institution Deemed to be University, At. & PO. Piparia, Tal. Waghodia, Dist. Vadodara -391760 (Gujarat), India

*Address for Corresponding Author

Dr. Ervilla Dass

Associate Professor,

Department of Pharmacology, Smt. Bhikhiben Kanjibhai Shah Medical Institute & Research Centre, Sumandeep Vidyapeeth an Institution Deemed to be University, At. & PO. Piparia, Tal. Waghodia, Dist. Vadodara -391760 (Gujarat), India


Abstract

Objective: Oxidative stress and diminished glutathione play critical roles in the pathogenesis of liver disease. Synthesis of glutathione, the most abundant mammalian antioxidant, is regulated at the substrate level by cysteine, which is synthesized from homocysteine via the trans-sulfuration pathway. Hence, the research study was aimed to explore the effects of single oral dose per se DL-Methionine. Material and Methods: Albino rats were separated into three groups for the experimental study. After overnight fasting, the albino rats belonging to Group I Control Group were administered Distilled Water 10 ml/kg orally; Group II Met. 700 mg/kg & Group III Met. 1400 mg/kg were administered DL-Methionine as a single oral dose by intragastric cannula. After 24-hours of post-treatment, serum level was evaluated to demonstrate the per se effects of DL-Methionine on liver. Also, the liver samples from each group were examined for the histopathological study. Results: The histopathological findings of DL-Methionine per se treated rats were compared with the control group. Gross examination revealed near to normal appearance & morphologic changes of liver tissue showed normal morphology and normal hepatic parenchymal cells. We observed that DL-Methionine as a single oral dose of 700 mg/kg and 1400 mg/ kg although caused alteration in levels of liver enzymes the alteration was not statistically significant. Conclusion: DL-Methionine per se although showed alteration in the biochemical parameters, they were not found to be significant so as to be considered as hepatotoxic agent. Also, Methionine is commonly used nutritional, essential amino acid, is preferred in the treatment of Paracetamol poisoning.

Keywords: DL-Methionine, liver function tests, biochemical changes, serum transaminase enzymes, histopathology

Manuscript Management System
Submit Article Subscribe Most Popular Articles Join as Reviewer Email Alerts Open Access
Our Another Journal
Another Journal
Call for Paper in Special Issue on

Call for Paper in Special Issue on