Research Articles

2018  |  Vol: 4(1)  |  Issue: 1(January-February)  |  https://doi.org/10.31024/ajpp.2018.4.1.11
Detection of siderophore production from different cultural variables by CAS-agar plate assay

Madhu Prakash Srivastava1*, Shashi Gupta2, Yogesh Kumar Sharma1

1Center of Excellence, Department of Botany, University of Lucknow, Lucknow-226 007, India

2K.G.B.V. Mohanlalganj, Lucknow, U.P., India

*Address for corresponding Author

Dr. Madhu Prakash Srivastava

Center of Excellence

Department of Botany, University of Lucknow, Lucknow-226 007, India

Abstract

Background: Siderophores are low molecular weight, Fe ion specific chelating agents which have been elaborated by fungi growing under low Fe stress. Trichoderma sp use the surrounding soil environment for nutrients primarily by hyphal extension. In this mode, Trichoderma sp secrete siderophores into the soil to bind tightly Fe that is subsequently bring back into the cell by specific uptake mechanism. Objective: This study was aimed to detection of siderophores production form different cultural variable by Chrome Azurol S (CAS)-agar plate assay in reference the rate of colour change of CAS medium as a function of time ( in days) when microorganism were grown  in CAS ager plate and also evaluate the effect of the inoculum type, iron concentration and pH. Material and methods: Fourteen strains of Trichoderma strains as MP1, MP2 ... and MP14 were selected for study. Out of theses the most Trichoderma MP1, Trichoderma MP3 and Trichoderma MP 9 were proved efficient among all strains, which exhibit higher reaction rate in CAS. Blue Agar assay in qualitative measurement of siderophore production. Results and conclusion: In the present study it was found that siderophore production in Trichoderma strains is an environment dependent phenomenon which is affect by different variable as pH and Fe concentration, as proved in in vitro conditions. They also exhibit their confirmed its best siderophore elaboration at 1mM concentration of fe and at pH 4.5.

Keywords: Siderophore, Chrome Azurol S, Trichoderma strains, in-vitro, iron

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