Krishna M. Shah1*, Vandana Mody2, Sunita S. Goswami1
1Department of Pharmacology, L. M. College of Pharmacy, Ahmadabad, Gujarat 380009 India
2Phyto Department, Cadila Pharmaceuticals Limited, Ahmedabad, Gujarat, 382225 India
*Address of Corresponding Author:
Krishna M. Shah
L. M. College of Pharmacy, Ahmadabad, Gujarat 380009 India
Background: Chronic unpredictable mild stress (CUMS) provokes behavioral alterations, oxidative-stress and neuroinflammation. CUMS mimics the clinical aspects of anxiety and depression in human beings. Existing antidepressant drugs possess slow onset of action, low response rates and problem of drug resistance. Therefore, there is a scope of alternative therapy for the treatment of neuropsychological illnesses. Objectives: The present study is aimed to assess the efﬁcacy of polyherbal formulation in CUMS model using mice and to explore the possible mechanism for the same. Materials and Methods: Mice were subjected to a series of stressful events for a period of 28 days. Drug treatments were given for a period of 28 days after the induction of disease. Parameters studied included behavioural aspects, sucrose preference test, brain neurotransmitters (5-HT, nor-adrenaline and dopamine) levels, serum pro-inflammatory cytokines (TNF-α, IL-1β and IL-1), corticosterone, quinolinic acid and oxidative markers. Results: Treatment with polyherbal formulation (400 & 800 mg/kg) significantly ameliorated behavioral deficits in several tests like forced swim test, tail suspension test, photoactometer and elevated plus maze model. It also reduced (p < 0.001) anhedonia using sucrose preference test. Significant up regulation of serotonin and other neurotransmitters along with reduction in oxidative stress was observed in treated animals. Further, polyherbal formulation also significantly attenuated the stress-induced increase in serum levels of TNF-α, IL-1β, IL-1, corticosterone and quinolinic acid. Conclusion: Our data suggest that this formulation enhances the neuroprotective effects against CUMS-induced oxidative stress, neuroinﬂammation and behavioral deﬁcits.
Keywords: Antidepressant, neuro-inflammation, oxidative-stress, neurotransmitters