Research Articles

2018  |  Vol: 4(4)  |  Issue: 4 (July- August)  |  https://doi.org/10.31024/ajpp.2018.4.4.11
Biological activity of phytoconstituents isolated from Cinnamomum zeylanicum and Punica granatum

Bindu I.1*, Elcey C. D.2

 1Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu, India.

2Department of Life science, Kristu Jayanti College (Autonomous), Bangalore-560077. Karnataka, India

*Address for Corresponding Author

Bindu I.

Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu, India

Abstract

Background: Ayurvedic preparations consist of a variety of medicinal plants with a range of properties. Cinnamomum zeylanicum Blume and Punica granatum L. are two plant species included in most of the preparations. The antioxidant property elicited by plant species has a full range of perspective applications in human healthcare. Plant-based risk-free, compounds with specific antioxidants and anticancerous activity is a continuous search. Objective: The present study aims to evaluate the purified extracts of the barks of C. zeylanicum and fruits peel of P. granatum so that they can be a potential source of molecules which has free radical scavenging or medicinal properties. Materials and methods: Plant extracts from C. zeylanicum bark and P. granatum fruits purified by using chromatographic methods. Structural elucidation of isolated compounds done by IR, HPLC and LC-MS analysis. The plant extracts were also evaluated for in-vitro antioxidant activity using  2,2'-AzinoBis [3-ethylbenzoThiazoline-6-Sulfonic acid]-di-ammonium salt (ABTS), nitric oxide and hydrogen peroxide radical with different concentrations of C. zeylanicum and  P. granatum extract. Results and conclusion: These assays revealed that the plant extracts possess inhibitory activity in a dose-dependent manner with IC50 values ranging from 19.32±1.62 to182.62 ±  0.38 μg/ml for ABTS assay, 771.10 ±  0.21 to > 1000μg/ml for nitric oxide assay and 278.64 ±  3.68 to 894.90± 4.38 μg/ml for hydrogen peroxide assay. In-vitro cytotoxicity of the purified compounds from plant extracts was tested against Vero (African green monkey kidney), A549 (Human lung carcinoma), MCF–7(Human breast cancer) and CaCo2 (Human colon carcinoma) cell lines. The result showed that CTC50 value of 61.55±4.8, 148.56±5.0, 75.11±3.4 and 113.00±2.5 by C. zeylanicum and 322.73±4.0, 599.01±4.0, 581.69±1.4 and 67.49±4.3 by P. granatum on Vero, A549, MCF – 7 and CaCo2 cell lines respectively.

Keywords: Cinnamomum zeylanicum Blume, Punica granatum, antioxidant, hydrogen peroxide, cytotoxicity

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