Research Articles

2019  |  Vol: 5(1)  |  Issue: 1(January-February)  |  https://doi.org/10.31024/ajpp.2019.5.1.2
Antibacterial activity of the hemolymph of Uca triangularis and its bacterial load

Sundaramurthy Kavitha1*, Jayaprakash Jayanthi.2, Bharathi Ravikrishnan3, Manickavalli Gurunathan Ragunathan 1, Shankar Sharmila1

1Department of Advanced Zoology and Biotechnology, Guru Nanak College, Velachery, Chennai-42, India

2Deputy Director, G.S.Gill Research Institute, Guru Nanak College, Velachery, Chennai-42, India

3Department of Biotechnology, Guru Nanak College, Velachery, Chennai-42, India

*Address for Corresponding Author

Sundaramurthy Kavitha

Department of Advanced Zoology and Biotechnology, Guru Nanak College, Velachery, Chennai-42, India

Abstract

Background: Crustacean is very important phylum, whose commercial outputs have not been understood clearly. In the present study the innate immune response among the male and female species of Uca triangularis was studied. Objective: To understand the immune response generated in Uca triangularis and to check if there is any difference in immune response among the male and female speciemen. Material and methods: Four different types of bacteria were isolated and identified from the hemolymph of both male and female healthy Uca triangular is, collected from the coast of Marina beach, Chennai. The bacteria were identified as E. coli, Staphylococcus aureus, Pseudomonas aeruginosa and Proteus vulgaris based upon their biochemical characterization and their growth pattern upon the selective media. The enzymatic biomarkers like glutathione-S transferase, glutathione reductase, super oxidase dismutase and non-enzymatic stress parameter such as reduced glutathione were estimated. The total hemocyte cell count in both male and female sample were estimated. Further the crude hemolymph preparation was subjected to the analyses of antibacterial activity by well diffusion technique. Results and conclusion: The maximum sensitivity pattern was observed against Staphylococcus aureus (24mm ± 0.0025- male hemolymph and 21mm ± 0.0037- female hemolymph) and the least inhibition was observed for Proteus vulgaricus (9mm ± 0.0015- male hemolymph and 5mm ± 0.0017- female hemolymph) was observed. It is evident from the present study that Uca triangularis harbors bacteria as commensal and its hemolymph contains antibacterial peptides, which prevents the bacteria becoming pathogen to the animal and the male hemolymph generates maximum inhibitory activity than female sample.

Keywords:  Uca triangularis, bacteria, hemolymph, antibacterial activity

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