Nabanita Medhi1*, Uma Dutta2
1Post-Graduate Department of Zoology, Darrang College, Tezpur, Assam, India
2Department of Zoology, Cotton University, Guwahati, Assam, India
*Address for Corresponding Author
Address: Post Graduate Department of Zoology,
Darrang College, Tezpur, District-Sonitpur, Assam, India, Pin-784001
Objective: The present study was carried out with an objective to evaluate the protective effect of methanolic extract of Aloe vera leaf gel and Bryophyllum pinnatum leaf against smokeless tobacco Gutkha (STG) induced toxicity in the kidney of Albino mice. Materials and Methods: The Swiss albino mice were divided into six (6) groups, among which two groups were treated with 100mg/kg and 200mg/kg body weight doses of STG orally for 90 days. Another three groups of mice were given STG along with A. vera and B. pinnatum leaf extracts singly (300 mg/kg) b. wt and in combination (mixture of both plant in 1:1 proportion) at an interval of 10 hours. Changes in kidney were evaluated at histological (LM and SEM level) and biochemical (SOD, CAT and MDA) level by following standard procedures. Results: LM analysis of histological structure of kidney revealed swelling and individualization of tubular epithelial cells, glomerular proliferation along with atrophied and enlarged glomerulus in STG treated mice. SEM analysis of surface structure of kidney of STG treated animals showed heterogenous surface morphology with reduced, necrosed and individualized tubules of kidney. Significant (p<0.01) alterations were also observed in selected enzyme parameters of STG treated mice. However, such histological and biochemical changes were not observed in A. vera and B. pinnatum extract treated animals. Conclusion: Result of the present study suggested the ameliorative potential of A. vera and B. pinnatum methanolic extract against STG induced nephrotoxicity with more efficacy of combined mixture than their individual application.
Keywords: Smokeless tobacco, Aloe vera, Bryophyllum pinnatum, nephrotoxicity