N. V. L. Suvarchala Reddy V*., Anjani M., M. Ganga Raju
Department of Pharmacology, Gokaraju Rangaraju College of Pharmacy, Bachupally, Hyderabad, Telangana, India
*Address for Corresponding Author
N.V.L. Suvarchala Reddy V.
Senior Assistant Professor,
Department of Pharmacology, GRCP, Bachupally, Hyderabad, Telangana, India
Objective: The present study was undertaken to evaluate nephroprotective activity of ethyl acetate and methanolic extract of the fruits of Illicium verum against paracetamol and gentamicin induced toxicity using male Wistar rats. Material and Methods: Randomly selected animals were divided into five groups of six animals each. The test extracts were administered orally at a dose of 200 mg/kg and 400 mg/kg. Nephrotoxicity was induced in rats by Paracetamol and gentamicin. The effect of extracts of Illicium verum at doses 200-400 mg/kg.b.wt compared with standard; was determined using serum urea, creatinine and uric acid. Furthermore, the effect of these extracts on some renal antioxidant enzymes and histopathological examination of kidneys were examined. Results: Paracetamol and gentamicin produced significant biochemical (increase in blood urea, serum creatinine, and serum uric acid level) changes, histological (damage to nephrons) changes, induced by paracetamol and gentamicin in kidney parameters. Pretreatment with Illicium verum extract significantly (p<0.005) prevented the physical, biochemical, and histological changes induced by paracetamol and gentamicin in the kidney. Conclusion: The ethyl acetate and methanolic extract of the fruit Illicium verum at two different dose level (200 & 400 mg/kg, bd.wt) was found to possess significant nephroprotective activity against paracetamol and gentamicin induced toxicity.
Keywords: Illicium verum, nephroprotective, paracetamol, gentamycin, antioxidant