Priyanka Jain, Vandana Soni*
Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar 470 003, Madhya Pradesh, India.
*Address for Corresponding Author
Prof. Vandana Soni,
Department of Pharmaceutical Sciences,
Dr.Hari Singh Gour, Central University, Sagar, Madhya Pradesh, 470 003, India.
Objective: The aim of this study was to investigate the targeting potential of transferrin anchored solid lipid nanoparticles (SLN) loaded with doxorubicin (D-SLN-T). Material and methods: Transferrin receptors (TR) are highly expressed on blood brain barrier as well as brain cancer cells. Therefore, targeting TR using transferrin (Tf) improved the anticancer activity of prepared nanoparticles. The Tf coupled SLN were characterized by fourier transform infrared spectroscopy, transmission electron microscopy, particle size, particle size distribution, zeta potential, % entrapment efficiency, vitro drug release and cell line studies. Results and conclusion: The average particle size of the D-SLN-T was found to be 210.3±1.3 nm with a negative surface charge. The cell line studies, on U87 MG brain cancer cell lines, showed that the cytotoxicity of D-SLN-T was highly increased when anchored with transferrin. Also, the presence of transferrin on the surface of the D-SLN-T enhanced the cellular uptake of drug on U87MG cell line. The results of the cytotoxicity and cellular uptake studies clearly showed the potential of D-SLN-T in brain cancer treatment.
Keywords: Transferrin, brain, solid lipid nanoparticles, doxorubicin, drug delivery