Jayanna Kengaiah, Sharath Kumar M. Nandish, Chethana Ramachandraiah, Chandramma Srinivasa, Ashwini Shivaiah, Devaraja Sannaningaiah*
Department of Studies and Research in Biochemistry and Centre for Bioscience and Innovation, Tumkur University, Tumkur, 572103, India
*Address for Corresponding author
Devaraja S., Ph. D.
Department of Studies and Research in Biochemistry,
Tumkur University, B H Road, Tumkur-572103 India
Objective: This study emphasizes antioxidant, anticoagulant and antiplatelet activities of Tamarind Seed Edible Extract (TSEE). Materials and Methods: Antioxidant activity was assessed by DPPH, Determination of oxidative stress marker, lipid peroxidation, Protein carbonyl content, and antiplatelet activity was tested using plasma recalcification time, bleeding time, APTT and PT Assays. Antiplatelet activity was studied by platelet aggregation studies using agonists ADP, Epinephrine and Collagen. Results: The TSEE showed similar protein banding pattern from the range of molecular mass 100kDa-14kDa under both reduced and Non-reduced conditions. It was signifying that the presence of monomeric protein in the extract. The TSEE showed proteolytic activity as it hydrolyzed casein and also the major role in the interference of plasma coagulation cascade and it induces clotting time. The TSEE extract was investigated for its role on platelet function, Surprisingly, TSEE inhibited the agonist ADP, Epinephrine and collagen-induced platelet aggregation in a dose-dependent manner and oxidative stress induced stress marker alteration in washed platelets is one among the many causes for the progression and development of thrombotic disorder like Alzheimer disease, arthritis, and diabetes millets and many chronic inflammatory disorders. TSEE were majorly used to determine the protective properties against the oxidative stress induced stress markers such as ROS, intracellular Ca2+ level, PCC, LPO, and thiols status. Conclusion: The TSEE shows the beneficial agent to protect the platelets from AAPH induced oxidative stress condition as well as agonist-induced platelet aggregation and its associated complication. Hence it considering the said facts that TSEE may be a better contender in the treatment of coagulation and haematological and cardiovascular disorders.
Keywords: Tamarind seed edible extract, antiplatelet, anticoagulant, antioxidant