Karan Singh1, Pathan Mohd Arif1, Mohd Imran Anees2, Mazahar Farooqui3, Samreen Fatema1*
1Post Graduate and Research Center, Maulana Azad College, Aurangabad (MS), India 431001.
2Y.B Chavan College of Pharmacy, Aurangabad (MS) India 431001.
3Dr. Rafiq Zakaria College for Women, Navkhanda, Aurangabad (MS) India 431001.
Address for Corresponding author:
Post graduate and research centre, Maulana Azad College, Aurangabad, (MS) India 431001
Objective: The main theme of the present study is that, to compare the other method of extraction from supercritical fluid extraction. Material and methods: The leaves of citrus are used for the investigation of inorganic compounds in the form of total ash. The leaves of citrus are also used for the latest extraction technique such as supercritical fluid extraction and the phytochemicals are present in it are compared with other method of extraction. The leaves and its extracts were used for fluorescent test, ash analysis, extraction, phytochemicals analysis, spectral analysis and antibacterial investigation. Results and Conclusion: The florescent test gives different color in different solvents. Ash analysis was gives about 15% of total ash. The samples were extracted using three different methods out of which SFE is found to be more effective. Phytochemical such as alkaloids, carbohydrate, phenolic compounds and tannins were present. All the extracts of citrus leave possess less antibacterial activity as compare with standard. Out of which SFE extract is moderately active against gram +ve bacteria. While all the extracts are showing less antibacterial activity against gram –ve bacteria. Conventional extract is inactive against all bacteria. This may be due to the degradation of flavonoids due to continue reflux, which possess antibacterial activity.
Keywords: Supercritical fluid extraction, Citrus leaves, phytochemicals, physicochemical, antibacterial activity