R. Aishwarya, Hema Kumari, R. Padmavathi*
Department of Pharmacology, G. Pulla Reddy College of Pharmacy, Hyderabad, Telangana, India
*Address for Corresponding Author
Dr R. Padmavathi
Department of Pharmacology,
G. Pulla Reddy College of Pharmacy, Hyderabad – 500028, Telangana, India
Objective: The present study was conducted to investigate that aqueous extract of Citrullus lanatus rind (CLRE) would ameliorate the hepatic damage caused by Ethanol. Materials and Methods: Thirty male wistar rats were divided into 5 groups as follows, Group-1 served as Control group, Group-2 as Disease control (20 ml/kg of 40% Ethanol on 28th day) and Groups-3, 4, 5 served as treatment groups 100 mg/kg, 200 mg/kg, 400 mg/kg of CLRE for 27 days and on 28th day 20 ml/kg of 40% Ethanol respectively. The hepatoprotective activity was evaluated by determining the liver serum parameters i.e., alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), anti-oxidant parameters like superoxide dismutase (SOD) and catalase (CAT), oxidative stress parameters - Malondialdehyde (MDA) and histopathological examinations. Results: Ethanol toxicity led to significant increase (P<0.001) of serum levels of AST, ALT, ALP and increased levels of MDA and decreased levels of SOD, catalase with multiple histological damage. Interestingly, Pre-treatment with CLRE, dose-dependently and significantly attenuated the changes caused by Ethanol administration. Conclusion: This study reveals that CLRE possesses hepatoprotective activity against 40% ethanol in dose dependent manner in rats.
Keywords: Citrullus lanatus rind, hepatotoxicity, ethanol, hepatoprotective, malondialdehyde