Sushama Dilip Patil1, 2*, Rahul Somani1, Ashish Jain2
1STE’s Smt. Kashibai Navale College of Pharmacy, Kondhwa (BK), Pune, Maharashtra, India
2Shri. D.D. Vispute College of Pharmacy and Research Center, Panvel, Maharashtra, India
Address for Corresponding author*
Sushama Dilip Patil
STE’s Smt. Kashibai Navale College of Pharmacy, Kondhwa (BK), Pune, Maharashtra, India
Background: Diabetes mellitus type II is the most prevalent metabolic disorder throughout the world and needs continuous development of pharmacotherapeutics for prophylaxis and treatment. Flavonoids are well known for their multifactorial mechanisms to treat diabetes mellitus. Dikamali, a gum resin from Gardenia gummifera is well known to contain the bunch of flavonoids. Objective: The present study was aimed to obtain the flavonoid-rich extract from the gum resin of Gardenia gummifera and evaluate its efficacy in streptozotocin-nicotinamide induced type II diabetic Wistar rats. Materials and methods: Evaluation of antidiabetic activity was done at the different dose level of extract viz. 100, 200 and 400 mg/kg body weight of an animal. Parameters such as fasting blood glucose, insulin, Insulin resistance (IR), food and water intake, body weight, lipid profile, urea, creatinine and, antioxidant status of organs were checked. Gluconeogenic enzymes activity was also estimated. Results: Studies indicated that the test extract reduced the fasting blood glucose and improved the glucose tolerance in diabetic rats by mitigating insulin resistance, lipid imbalance, and oxidative stress. The test extract also alleviated the enhanced activities of gluconeogenic enzymes in diabetic rats. The micrographs of pancreas histopathology were also supportive of the above findings. The extract also reduced the urea and creatinine levels in diabetic rats which are indicative of its potential in treating diabetic complications also. Conclusion: Overall, the test extract showed attractive findings and thus in future, it can be a promising candidate for developing alternate therapeutic moiety for the treatment of diabetes, hyperlipidemia and associated complications.
Keywords: Antidiabetic, Gardenia gummifera, streptozotocin, nicotinamide, flavonoids