Amit Verma, Ankit Jain, Ankita Tiwari, Sanjay K. Jain*
Pharmaceutics Research Projects Laboratory, Department of Pharmaceutical Sciences, Dr. Hari Singh Gour Vishwavidyalaya, Sagar (M.P.), India – 470 003
Professor Sanjay K. Jain,
Pharmaceutics Research Projects Laboratory,
Dean, School of Engineering and Technology, Department of Pharmaceutical Sciences,
Dr Harisingh Gour Vishwavidyalaya, Sagar (M.P.), India - 470 003
Background: Natamycin (NAT) is a broad-spectrum polyene antifungal agent employed in the treatment of fungal keratitis, blepharitis, and conjunctivitis. It is specially used against ﬁlamentous fungi due to its better safety proﬁle, and transcorneal penetration potential. Objective: To assay the physicochemical properties of natamycin and development of its niosomal formulation. Material and Methods: The natamycin was characterized for its meting point, solubility profile and partition coefficient, which were almost similar as standard. Natamycin was insoluble in almost organic and inorganic solvents. Fourier Transform Infrared (FTIR) and UV-Visible spectroscopy were also performed and the spectra for both spectroscopies were almost superimposable with previously reported spectra. The Differential Scanning Calorimetry (DSC) study gave an idea about the compatibility of natamycin with other formulation additives. Thin Film Hydration (TFH) method was employed for the development of natamycin loaded niosomal formulation and it was characterized for vesicle size, zeta potential, % entrapment efficiency (% EE) and percentage drug release up to 12 hrs. Results and conclusion: In preformulation studies the absorption maxima for drug was found to be 317 nm. The drug is insoluble in almost all solvents, but it was showing solubility only in acetic acid, acidic methanol and mixture of chloroform-methanol (3:2). The melting point of drug was found to be 290-295 °C, which was indicating the thermal stability of drug during entire range of temperatures for formulation development. The FTIR spectrum of the drug is almost similar to the standard which indicates the purity and stability of drug. The vesicle size, zeta potential and % EE of the developed niosomes were found to be 968±8.24 nm, 22.81±2.19 mV and 78.61±3.09 % respectively. The % drug release was found to be 87.66±4.32 % in 12 hrs. The results of all parameters are acceptable and can be utilized for further studies.
Keywords: Natamycin, Niosomes, physicochemical characterization, solubility, UV spectroscopy, FTIR spectroscopy