R. Selvam, K. Muruganantham, S. Subramanian*
Department of Biochemistry, University of Madras Guindy Campus, Chennai-600025 Tamilnadu, India
*Address for Corresponding Author
Dr. S. Subramanian
Department of Biochemistry
University of Madras, Guindy Campus, Chennai-600025, Tamilnadu, India
Background: Type 2 diabetes mellitus (T2DM) is an endocrine disorder characterized by insulin resistance, β cell dysfunction, visceral obesity, inflammation and chronic oxidative stress. However, insulin resistance is predominant in type 2 DM which results in persistent hyperglycemia. Chronic hyperglycemia promotes auto-oxidation of glucose to form free radicals. The generation of free radicals beyond the scavenging abilities of endogenous antioxidant defenses results in the development of both macro- and microvascular complications. Objective: In present study, we have evaluated the antioxidant property of swertiamarin, a secoiridoid glycoside present in the Enicostemma littorale blume leaves in High fat fed and -Low dose streptozotocin induced type 2 diabetic rats. Materials and Methods: High-fat diet-fed and low-dose STZ was used to induce experimental type 2 diabetes in rats. Diabetic rats were orally treated with swertiamarin (50 mg/kg b.w./rat/day) for 30 days. The levels of oxidative stress markers such as lipid peroxides, hydroperoxides and protein carbonyls were determined. The levels of both enzymatic as well as non enzymatic antioxidants were evaluated. Results and conclusion: Diabetic rats showed significantly increased levels of oxidative stress markers and declined levels of both enzymatic and non enzymatic antioxidants. Oral treatment of swertiamarin at a concentration of 50mg/kg bw/rat/day for 30 days decreased the levels of oxidative stress markers and improved the status of antioxidants in diabetic rats indicating the antioxidant properties of swertiamarin in addition to its antidiabetic properties.
Keywords: Swertiamarin, high fat, oxidative stress, antioxidant