Priyal Patel1*, Jayvadan Patel2
1Babaria Institute of Pharmacy, BITS Edu Campus, Vadodara, Gujarat, India
2Nootan Pharmacy College,Visnagar,Gujarat. India.
Priyal R. Patel
Department of Pharmaceutics
Babaria Institute of Pharmacy, BITS Edu Campus, Varnama, Vadodara, Gujarat, India
Objective: The purpose of this study was to develop freeze dried Polylactide (PLA) nanoparticles loaded with Mitomycin C intended to be administered intravenously with improved therapeutic efficiency of the drug and thereby reduced dose related toxicity associated with oncology drugs. Materials and methods: The Mitomycin C loaded nanoparticles were prepared using modified spontaneous emulsification solvent diffusion method (SESD) and nanoprecipitation method. The in Vitro Release study of Mitomycin C loaded Polylactide (PLA) –NP was carried by dialysis bag diffusion technique. Results and discussion: The incorporation efficiency was found to be higher in Modified SESD technique than emulsification solvent evaporation method. The release behavior of Mitomycin C exhibited a biphasic pattern characterized by an initial burst release followed by a slower and continuous release. The nanoparticles were characterized by particle size, zeta potential, polydispersity index, DSC and FTIR. The long term stability was achieved by lyophilisation technique. The cell line study using XTT assay on LNCaP prostate tumor cell lines revealed that Mitomycin C loaded nanoparticles showed greater cancer cell inhibition compared to plain nanoparticles and marketed conventional formulation.
Keyword: PLA, Mitomycin C, Lyophilization, Cryoprotectant, cell line studies, cytotoxicity studies