Ambadas Mane, Sachin Tembhurne*, Swati Kolhe
Department of Pharmacology AISSMS College of pharmacy, Savitribai Phule Pune University, Pune, Maharashtra, India
*Address for Corresponding Author
Dr. Sachin V. Tembhurne
Assistant Professor, Department of Pharmacology,
All India Shri Shivaji Memorial Society’s College of Pharmacy, Kennedy road, Near RTO, Pune 01, Maharashtra, India
Objective: In this study, we tested the effect of hydroalcoholic extract of Dalbergia sissoo on oxidative stress, lipid profile in 90 days postsurgical bilateral ovariectomized female rats. Material and Methods: 3-month old female Wistar rats were used and distributed in 6 groups: intact groups with 90 days, ovariectomized groups with 90 days, ovariectomized (Ovx) treated hydroalcoholic extract of Dalbergia sissoo (200, 350 and 500mg/kg p.o.), ovariectomized treated with standard β Estradiol (0.1mg/kg/s.c). All the treatments were given for further 90 days after post-surgical period (90 days) in ovariectomized female rats. After 90 days of treatments serum estrogen level, level of thiobarbituric acid reactive substances and lipid profile were quantified. Results: The treatment of the hydroalcoholic extract of Dalbergia sissoo (200, 350 and 500mg/kg) in the Ovx rats shows significant increase in the serum estrogen level, normalization of lipid profile, decreased in the thiobarbituric acid reactive substances. All the results of Dalbergia sissoo are comparable with standard β Estradiol. Conclusion: Our results suggest that 90 days of treatment with hydroalcoholic extract of Dalbergia sissoo is able to increase the estrogen level thereby normalized the lipid profile and oxidative stress in ovariectomized rats.
Keywords: Dalbergia sissoo, ovariectomized, estrogen, lipid profile, oxidative stress