Aruna K.*, Indra Sukesh
Department of Microbiology, Wilson College, Mumbai 400007, Maharashtra, India
*Address for Corresponding Author
Dr. K. Aruna
Head of Microbiology & Co-ordinator of Biotechnology Department.
Wilson College, Mumbai 400007 India
Objective: The emerging problem of antibiotic resistance has driven an urge among scientists to explore alternative treatment methods by pursuing the teachings of traditional medicine. Hence the current study was carried out to investigate the efficacy of Psidium guajava leaves extract as a possible alternative source of medicine against drug-resistant pathogens. Materials and methods: The leaves extract of P. guajava was prepared in ethanol and water using a Soxhlet apparatus and its antibacterial activity were tested against 50 representative isolates of Extended spectrum β-lactamase (ESBL) and Metallo-β-lactamase (MBL) producing uropathogens. Qualitative analysis of the antibacterial activity of P. guajava was carried out by well diffusion and agar diffusion method. Results: It was found that the ethanol extract of P. guajava leaves exhibit considerable antibacterial activity with the observed zones of inhibition in the range of 20-25mm against the test isolates. Water extract of P. guajava, on the other hand, showed comparatively smaller zones of inhibition i.e., in the range of 15-18mm. In addition, the ethanol extract of P. guajava showed an MBC of 30mg/mL for both ESBL and MBL producers. Furthermore, they showed synergistic activity with ampicillin antibiotic, which was indicated by decreased MBC of ampicillin (i.e., in the range of 200-300µg/mL from >10mg/mL) against ESBL and MBL producers in our study. Conclusion: Thus, P. guajava plant extracts showed promising results to be used as an alternative treatment source for infections caused by ESBL and MBL producing uropathogens.
Keywords: P. guajava, extended spectrum β-lactamase, Metallo-β-lactamase, synergy