Research Articles

2020  |  Vol: 6(6)  |  Issue: 6 (November-December)  |  https://doi.org/10.31024/ajpp.2020.6.6.6

Simultaneous quantification of Lupeol, β-Sitosterol in the extract of Terminalia tomentosa and Eugenol, Kaempferol in Syzygium cumini extract using GC-MS

Tanmay Chache^1*, Seema R. Sapale², Vikas Vaidya³, Deepal Ghadge¹

¹Ramnarain Ruia Autonomous College, L. N. Road, Matunga (East), Mumbai 400 019 Maharashtra, India

²Kirti M Doongursee College, Dadar, Mumbai 400028, Maharashtra, India

³Royal College of Arts, Science and Commerce, Mira Road (E), Dis- Thane 401107 Maharashtra, India

*Address for Corresponding Author

Tanmay Chache

Ramnarain Ruia Autonomous College,

L. N. Road, Matunga (East), Mumbai 400 019 Maharashtra, India

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Abstract

Objective: A precise, accurate and reproducible GC-MS method is validated for simultaneous quantification of two bioactive markers in Lupeol and β-Sitosterol in the plant of Terminalia tomentosa and Eugenol and Kaempferol in the plant of Syzygium cumini. Materials and Methods: The mobile phase used was Helium gas. The column used was Rtx-1ms (100% dimethylpolysiloxane).The flow rate for developing the method in T. tomentosa plant was 1.5ml/min while in S. cumini was 0.9 ml/min. Results and Conclusion: Two precise methods were developed for Simultaneous Quantification of two different plants using Lupeol, β-Sitosterol in the extract T. tomentosa plant and Eugenol, Kaempferol in the extract of S. cumini plant. The method can be used for routine quality control of herbal raw materials as well as formulations containing any or all of these compounds.

Keywords: GC MS, Simultaneous quantification, T. tomentosa, S. cumini