Research Articles

2019  |  Vol: 5(1)  |  Issue: 1(January-February)  |  https://doi.org/10.31024/ajpp.2019.5.1.26
GCMS analysis, antioxidant and antibacterial activities of ethanol extract of Anisomeles malabarica (L.) R.Br. ex. Sims leaves

Saraswathi Krishna1, Sivaraj Chandrasekaran2*, Dhivya Dhanasekar2, Arumugam Perumal2

1Karpaga Vinayaga College of Engineering and Technology Madhuranthagam, Kancheepuram – 603 308 India

2Armats Biotek Training and Research Institute, Maduvinkarai, Guindy, Chennai-600 032 India

*Address for Corresponding Author

Sivaraj C, Postdoctoral Fellow

Armats Biotek Training & Research Institute

Maduvinkarai, Guindy, Chennai - 600 032 India

Abstract

Objective: The leaves of Anisomeles malabarica belonging to the family Lamiaceae was evaluated for antioxidant and antimicrobial activities. Materials and methods: The ethanol extract of leaves of Anisomeles malabarica revealed the presence of alkaloids, phenols, steroids, glycosides, etc. The total phenolic content and total flavonoid content were carried out by Folin-ciocalteau reagent and AlCl3 reagent methods. The antioxidant activities of ethanol extract of Anisomeles malabarica were carried out by DPPH· radical, Superoxide radical (O2•-) and ABTS•+ radical cation scavenging assays, Phosphomolybdenum reduction and Fe3+ reduction assays. Results: The IC50 of DPPH˙ radical, Superoxide radical (O2•-) and ABTS•+ radical cation scavenging assays were 94.18, 65.31 and 40.84 µg/mL concentration respectively. Also, the RC50 of phosphomolybdenum reduction and Fe3+ reduction were 19.16 and 34.98 µg/mL concentration respectively. The antibacterial activity for ethanol extract of leaves of Anisomeles malabarica was carried out against bacterial pathogens such as Staphylococcus aureus, Bacillus subtilis, Proteus vulgaris. The major findings of GCMS analysis were Phenol, 2, 4-bis (1, 1-dimethylethyl)-, Oleic acid, Coumarine, 3-[2-(1-methyl-2-imidazolylthio)-1-oxoethyl]-  exhibiting significant therapeutic applications. Conclusion: The results provide justification for the uses of ethanol extract of Anisomeles malabarica to treat various infectious diseases. Further the ethanol extract could be turned as an effective drug for cancerous cells.

Keywords: Free radicals, ABTS·+ radical cation, superoxide radical (O2•-), RC50, agar well diffusion, GCMS

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