Research Articles

2019  |  Vol: 5(2)  |  Issue: 2(March-April)  |  https://doi.org/10.31024/ajpp.2019.5.2.8
Interaction of herbal formulation and conventional drug on anxiolytic potential in mice

Priya Mishra*, Pushpendra Kannojia1, Deeksha Sahai2, Mohd Asif Khan3

1Department of Pharmacology, IPS-College of Pharmacy, Shivpuri Link Road, Gwalior-474002 (M.P.), India

2,3Bareilly International University, College of Pharmacy, Bareilly-243006 (U.P.) India

*Address for corresponding author

Mohd Asif Khan

Assistant Professor,

Bareilly International University,

BIU College of Pharmay, Bareilly- 243006 (UP) India

Abstract

Objective: The present study was designed to evaluate the interaction among the herbal formulation with the conventional drugs in the treatment of anxiety in rodents. Present study deals with the pharmacodynamics interaction of conventional Diazepam with Ashwagandha churna. Material and Methods: Interaction was studied by the influence on the anxiolytic effect of the standard drug in Elevated plus Maze apparatus and light/dark model induced anxiety in Swiss albino mice (male) weighing between (28-35gm). The study also includes the standardization of selected herbal formulation with various parameters like Ash value, extractive value, moisture content, pH and phytochemical investigation. Acute toxicity studies (50mg/kg, 300mg/kg and 2000 mg/kg p. o.) were conducted to determine the safe dose as per OECD-423 guidelines. Four Groups of animals (n=6) were used in this study. Group I was disease control group, Group II received Ashwagandha churna, Group III received Diazepam and Divya ashwagandha churna and Group IV kept as standard drug (Diazepam) treated. Results and Conclusion: No sign of toxicity was observed. The test sample increases the time spent and number of entries in open arms but the combination of Diazepam and test herbal formulation decreases the time spent and number of entries in open arms in E.P.M. Data of this method was analysed by annova and dunnetts multiple comparison at p<0.01. In conclusion, the results suggest that there exist an interaction between conventional Diazepam and herbal formulation which was a negative type.

Keywords: Anxiolytic, Ashwagandha churna, diazepam, light/dark arena

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