Sachin Bhusari*, Shivani Bhokare, Kanchan Nikam, Pravin Wakte
Pharmaceutical Technology Division, Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India
*Address for Corresponding author
Dr. Sachin Shivling Bhusari,
Pharmaceutical Technology Division,
Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad-431001, Maharashtra, India
Objective: To evaluate pharmacognostic and phytochemical analysis of leaves of Cissus repanda, including macroscopic, microscopic and physicochemical parameters. Material and Methods: Macroscopic and microscopic properties of dried leaves were investigated. Preliminary phytochemical analysis of various extracts and fluorescent analysis of dried leaf powder was performed using various chemicals. Physicochemical parameters were evaluated as per WHO guidelines. Results: Organoleptic parameters Cissus repanda leaves like colour, shape, size etc were noted. Transverse section of fresh leaves revealed the presence of collenchyma, non-lignified xylem and phloem cells and Calcium oxalate crystals in mesophyll cells. Whereas, powder microscopy showed the presence of Trichome, xylem parenchyma cells, lignified cells, crystals of calcium oxalate and Helical Xylem Vessel. Phytochemical screening of ethanolic extract of Cissus repanda leaves showed presence of alkaloids, flavonoids, glycoside, steroids and carbohydrates. Hydro-alcoholic extract showed the presence of glycoside, flavonoids, tannin and steroid while its aqueous extract showed presence of glycoside, tannins and flavonoid. Physicochemical parameters viz. ash value, extractive value, moisture content and fluorescent analysis of leaf powder were determined. Conclusion: The present study revealed pharmacognostic and phytochemical parameters of Cissus repanda leaves which would be beneficial for its standardization and future scope of the work.
Keywords: Cissus repanda, pharmacognostic study, phytochemical analysis