Gita Mishra1, Hemeshwer Kumar Chandra1, Nisha Sahu1, Satendra Kumar Nirala2, Monika Bhadauria*1
1Toxicology and Pharmacology Laboratory, Department of Zoology, Guru Ghasidas University, Bilaspur, Chhattisgarh, India.
2Department of Rural Technology and Social Development, Guru Ghasidas University, Bilaspur, Chhattisgarh, India.
Dr. Monika Bhadauria
Toxicology and Pharmacology Laboratory
Department of Zoology, Guru Ghasidas University, Bilaspur, 495009 (C.G.) India
Objective: Pergularia daemia (Forsk.) of Asclepiadaceae family is well known for its hepatorenal protective ability, however very less data is available for its safety evaluation and choleretic activity. The present study was performed to investigate no-observed-adverse-effect level (NOAEL) and choleretic activity of P. daemia leaves extract. Materials and Methods: P. daemia leaves extract was orally administered at 100, 200, 400 and 800 mg/kg dose to female wistar rats for seven days. Blood was collected and serum biochemical parameters were performed after 24 h of last treatment. Evaluation of choleretic activity was done by intraduodenal administration of extract at 400 mg/kg followed by bile collection. Results: There were no significant change in alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, total bilirubin, albumin, urea, uric acid, creatinine, triglycerides and cholesterol. Slight increase in bile volume and bile solid contents was found in tested and dihydrocholic acid (DHC) treated standard group compared to control. Conclusion: P. deamia leaves extract is safe for in vivo studies in rats (NOAEL 800 mg/kg) and it also possess choleretic potential.
Keywords: Pergularia daemia, No-observed-adverse-effect level, cholerectic activity