Mithun Mukherjee*, Sharmistha Gupta
West Bengal State Council of Science and Technology, Vigyan Chetana Bhawan, Salt lake, Kolkata-64 India
*Address for Corresponding Author
Department of Science and technology
Government of West Bengal, Vigyan Chetana Bhavan
Plot No. 26/B. Block DD. Sector-1, Salt Lake, Kolkata-64 India
Objective: Evaluation of Acute toxicity and Anti-asthmatic activity of the ethyl acetate extract from the leaves of Phyllanthus niruri (L.) were studied as per OECD guideline 423. Materials and Method: Anti -asthmatic activity of the extract was investigated against compound 48/80 and egg albumin induced mast cell degranulation. Percentage mast cell degranulation was calculated at different concentration level i.e.1, 10, 100 and 1000μg/mL against compound 48/80 and at 200mg/kg body weight of dose P.O. against egg albumin. Results: The leaf extracts showed significant protection of rat mesenteric mast cells from disruption caused by compound 48/80 and egg albumin. Significant dose dependent effect was observed in percentage mast cell degranulation at different dose level of compound 48/80 and egg albumin in comparison to negative control at 30.88 ± 0.50 and at 38.26 + 1.27 mast cell degranulation was observed at 1000μg/mL and 200 mg/kg body dose level of the leaf extracts. Conclusion: The present study revealed that the Phyllanthus niruri L. leaf extracts of the ethyl acetate fraction has significant anti-asthmatic activity against compound 48/80 -induced mast cell degranulation as well as that induced by egg albumin in comparision to that produced by Ketotifen fumarate. There was no lethal and toxic reactions found in the tested animals.
Keywords: Acute oral toxicity, compound 48/80, egg albumin, mast cell degranulation, Ketotifen fumarate