Research Articles

2019  |  Vol: 5(5)  |  Issue: 5(September- October)  |  https://doi.org/10.31024/ajpp.2019.5.5.30
Investigation for inhibitory effect Uca triangularis extracts

Sundaramurthy Kavitha1, Jayaprakash Jayanthi2, Bharathi Ravikrishnan3, Shankar Sharmila1, Manickavalli Gurunathan Ragunathan1

1Department of Advanced Zoology and Biotechnology, Guru Nanak College, Velachery, Chennai-42, India

2G.S.Gill Research Institute, Guru Nanak College, Velachery, Chennai-42, India

3Department of Biotechnology, Guru Nanak College, Velachery, Chennai-42, India

Address for Corresponding Author:

Dr. M. G. Ragunathan,

Associate Professor and Head, Department of Advanced Zoology and Biotechnology, Guru Nanak College, Velachery, Chennai-600042 India

Abstract

Objective: There are increased incidences of the multi-drug resistance Staphylococcus aurues infections, worldwide, especially in India. In India among the male population the prostate Cancer is high. Thus it results in high mortality rate. Hence this present research is aimed to identify the alternative compounds which generate effective microcidal properties and also the anticancerous properties against the prostate cancer cell line model- PC3. Material and Method: The hemolymph of both male and female crab Uca triangularis was extracted and screened for the antibacterial activity against Staphylococcus aureus and same the same hemolymph of the male and female was subjected to protein purification by ammonium sulphate precipitation, combination of molecular sieve and ion exchange chromatography and the purified protein fraction was subjected to cytotoxicity assay against PC-3 cell line. Result and Conclusion: The crude male hemolymph sample was found to generate better antibacterial activity against Staphylococcus aureus as 9mm ± 0.02 at 50µl by the well diffusion method. The DEAE cellulose- Sephadex A-25 generated antibacterial activity against Staphylococcus aureus as 11 mm ± 0.24.  The anticancerous study against PC3 cell line, the IC50 value was determined 120µg/ ml and 29.6% ± 0.87 cells were found to be viable at 200 µl/ ml of the male hemolymph fraction. The antimicrobial peptides extracted from the Uca triangularis of the both male and female was able to generate the both antibacterial and anticancerous properties.

Keywords: Uca triangularis, antimicrobial peptides, anticance compounds

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