Research Articles

2020  |  Vol: 6(4)  |  Issue: Articles in press  |  https://doi.org/10.31024/ajpp.2020.6.4.2
Bioactive compound isolated from marine Bacillus safensis MB8 and their cytotoxicity potential

Lalitha Perumal*

Department of Biotechnology, Pondicherry University, Kalapet, Puducherry-605014, India

*Address for Corresponding Author

Prof. Lalitha Perumal

Department of Biotechnology,

Pondicherry University, Kalapet, Pondicherry-605014, India


Abstract

Background: Marine bacteria represent a potential source for the production of medically useful compounds against cancer, diabetes, inflammation, infection, etc. Objectives: In the present study, isolation, characterization and identification of bioactive compound from marine Bacillus safensis MB8.  Methods: Breast cancer (MCF-7) cell lines to determine the cytotoxicity activity of bioactive compound produced from Marine Bacillus safensis MB8. Results: Marine Bacillus safensis MB8 (Genbank accession no. KJ531643) isolated from deep sea sediments of the Bay of Bengal, India, produced a bioactive compound, Bis (2-ethylhexyl) benzene-1,2-dicarboxylate (BEHBD) with a molecular formula of C24H38O4. The molecular ion was m/z 391 (M+). The MTT assay based cytotoxicity assessment of BEHBD in MCF-7 cancer cells revealed the IC50 to be 49.8µg/ml.  The acridine-orange and ethidium bromide (AO/EB) staining of the BEHBD treated cancer cells showed typical characteristic of apoptosis such as nuclear condensation, cell shrinkage and formation of apoptotic bodies. Conclusion: Potent cytotoxicity compound of Bis (2-ethylhexyl) benzene-1, 2-dicarboxylate (BEHBD) produced from marine Bacillus safensis MB8. It was undoubtedly confirmed to BEHBD is a proved cytotoxicity compound. This is the first reported to BEHBD produced by marine Bacillus safensis MB8.

KeywordsMarine bacteria, Bacillus safensis, Bis (2-ethylhexyl) benzene-1,2-dicarboxylate, MCF-7, Cytotoxicity activity, AO/EB staining

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