Research Articles

2018  |  Vol: 4(6)  |  Issue: 6 (November-December)  |  https://doi.org/10.31024/ajpp.2018.4.6.4
Forskolin, Ameliorates Mitochondrial Dysfunction in Streptozotocin induced diabetic Nephropathy in rats

Surbhi Rana, Lakhwinder Singh*, Sidharth Mehan3

1Research Scholar, I. K. Gujral Punjab Technical University, Jalandhar, Punjab, India  

2Head Department of Applied Science CGC College of Engineering, Landran, Punjab, India

3Associate Professor, Department of Pharamcology, ISF College of Pharmacy, Moga, Punjab, India

*Address for Corresponding Author

Lakhwinder Singh

Head Department of Applied Science CGC College of Engineering,

Landran, Punjab, India-140307

Abstract

Objective: Diabetes downregulates the expression of nitric oxide and elevate the reactive oxygen species resulting in oxidative stress leads to mitochondrial dysfunction. In the present study we have examined the role of Forskolin in reduced nitric oxide and mitochondrial dysfunction, which may be responsible in controlling diabetic nephropathy. Materials and methods: Diabetes mellitus in rats was induced by feeding high fat diet (HFD) for 2 weeks followed by single low dose of Streptozotocin (STZ) 35mg/kg, i.p. further followed by high fat diet for next ten weeks. Streptozotocin administered rats exhibited apparent renal functional changes as compared to normal rats. These renal functional abnormalities were complimented with increased uric acid, liver functions and mitochondrial complexes dysfunction as assessed in terms of mitochondrial Adenosine triphosphate (ATP) levels in Liver, Kidney and Pancreas, Alkaline phosphatase (ALP), succinate dehydrogenase (SDH), Nicotinamide adenine dinucleotide phosphate (NADPH), Lactate dehydrogenase (LDH). Results: Two weeks treatment with Forskolin presented a marked renoprotection by significantly preventing renal abnormalities supported by biomarkers of mitochondrial and liver dysfunctions tests. Conclusion: Hyperglycaemia produces mitochondrial dysfunction with dysregulation between oxidative stress and nitric oxide. Diminished nitric oxide level, elevated uric acid, liver and mitochondrial dysfunction could play critical role in Streptozotocin induced mitochondrial dysfunction in diabetic nephropathy. This study provides preliminary evidences that Forskolin markedly prevented mitochondrial dysfunction and progression of nephropathy.  

Keywords: Mitochondrial dysfunction, mesangial expansion, oxidative stress, Forskolin 

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